UBE2U Double Nickase Plasmid (h): sc-409509-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2U Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBE2U Double Nickase Plasmid (h) and UBE2U Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBE2U. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2U Double Nickase Plasmid (h) | sc-409509-NIC | 20 µg | $410.00 |
UBE2U encodes an E2 ubiquitin-conjugating enzyme that partners with E1 and E3 ligases to transfer activated ubiquitin to substrate proteins, thereby shaping proteostasis and ubiquitin-dependent signaling. Through regulation of ubiquitination, UBE2U can influence protein turnover, trafficking, and quality-control pathways that intersect with cell-cycle control and stress responses. Dysregulated ubiquitin pathway activity is broadly implicated in oncogenic signaling and neurodegeneration, making UBE2U a useful node for dissecting how ubiquitin conjugation modulates cellular homeostasis. Research on UBE2U supports mechanistic studies of ubiquitin-network wiring, substrate selection, and pathway crosstalk in human cells.
UBE2U Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBE2U locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBE2U. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBE2U function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBE2U-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.