UBE2D2 Antibody (Y-20): sc-100617

UBE2D2 Antibody (Y-20) is a mouse monoclonal IgG2a kappa light chain antibody that detects UBE2D2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-UBE2D2 antibody (Y-20) is available as a non-conjugated format. UBE2D2 plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. UBE2D2 is primarily located in the cytoplasm, where UBE2D2 interacts with various E3 ligases to facilitate the ubiquitination process. UBE2D2′s cytoplasmic localization enables quick responses to cellular stress and regulation of misfolded or damaged protein degradation, preventing potentially toxic aggregate accumulation. UBE2D2 catalyzes IκBα ubiquitination in a phosphorylation and SCFβ-TRCP-dependent manner, where E1 first transfers ubiquitin to UBE2D2, which then associates with E3 ligase to conjugate a poly-ubiquitin chain onto target proteins. This tagging process is vital for IκBα degradation by the proteasome, which subsequently allows NFκB to translocate to the nucleus and activate genes involved in immune and inflammatory responses.

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UBE2D2 Antibody (Y-20) References:

1. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.  |  Gong, L. and Yeh, ET. 1999. J Biol Chem. 274: 12036-42. PMID: 10207026
2. Heat-induced cell cycle arrest of Saccharomyces cerevisiae: involvement of the RAD6/UBC2 and WSC2 genes in its reversal.  |  Raboy, B., et al. 1999. Mol Microbiol. 32: 729-39. PMID: 10361277
3. SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4.  |  Strack, P., et al. 2000. Oncogene. 19: 3529-36. PMID: 10918611
4. MUL1-RING recruits the substrate, p53-TAD as a complex with UBE2D2-UB conjugate.  |  Lee, MS., et al. 2022. FEBS J. 289: 3568-3586. PMID: 35048531
5. Circular RNA-UBE2D2 accelerates the proliferation and metastasis of non-small cell lung cancer cells via modulating microRNA-376a-3p/Eukaryotic Translation Initiation Factor 4γ2 axis.  |  Tong, J., et al. 2022. Bioengineered. 13: 5942-5953. PMID: 35196197
6. Circ_UBE2D2 Attenuates the Progression of Septic Acute Kidney Injury in Rats by Targeting miR-370-3p/NR4A3 Axis.  |  Huang, Y. and Zheng, G. 2022. J Microbiol Biotechnol. 32: 740-748. PMID: 35722711
7. The cuproptosis-related gene UBE2D2 functions as an immunotherapeutic and prognostic biomarker in pan-cancer.  |  Fei, Y., et al. 2024. Clin Transl Oncol. 26: 2718-2737. PMID: 38703335
8. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
9. The ubiquitin-proteasome proteolytic pathway.  |  Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
10. The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins.  |  Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
11. The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme.  |  Schwarz, SE., et al. 1998. Proc Natl Acad Sci U S A. 95: 560-4. PMID: 9435231
12. A novel protein modification pathway related to the ubiquitin system.  |  Liakopoulos, D., et al. 1998. EMBO J. 17: 2208-14. PMID: 9545234

The preferred UBE2D antibody is UBE2D Antibody (C-6): sc-166278.