U2 SnRNP A Antibody (B-3): sc-393804
- U2 SnRNP A Antibody (B-3) is a mouse monoclonal IgG1 κ U2 SnRNP A antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-255 representing full length U2 snRNP A of human origin
- U2 SnRNP A Antibody (B-3) is recommended for detection of U2 snRNP A of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-U2 SnRNP A Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-393804 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP is the preferred secondary detection reagent for U2 SnRNP A Antibody (B-3) for WB applications. This reagent is now offered in a bundle with U2 SnRNP A Antibody (B-3) (see ordering information below).
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U2 SnRNP A Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects U2 SnRNP A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). U2 SnRNP A (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. U2 SnRNP A protein, also known as SNRPA1 or U2A, plays a crucial role in pre-mRNA splicing, essential for proper gene expression. U2 SnRNP A protein functions as a key component of U2 small nuclear ribonucleoprotein (snRNP) complex, involved in spliceosome formation that catalyzes intron removal from pre-mRNA. U2 SnRNP A protein interacts with U2 snRNP B to form a dimer binding to U2 snRNA hairpin IV, a critical step in splicing. This U2 snRNP A/U2 snRNP B complex structure, along with specific amino acid variations, determines snRNP binding specificity to RNA, highlighting U2 SnRNP A′s importance in gene regulation and expression. U2 SnRNP A consists of 255 amino acids, with two isoforms resulting from alternative splicing events, underscoring U2 SnRNP A′s significance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
U2 SnRNP A Antibody (B-3) References:
- The SRm160/300 splicing coactivator is required for exon-enhancer function. | Eldridge, AG., et al. 1999. Proc Natl Acad Sci U S A. 96: 6125-30. PMID: 10339552
- Gene Structure of the U2 snRNP-Specific A' Protein Gene from Salmo salar: Alternative Transcripts Observed. | Lundin, M., et al. 2000. Mar Biotechnol (NY). 2: 204-211. PMID: 10811961
- The Drosophila U2 snRNP protein U2A' has an essential function that is SNF/U2B' independent. | Nagengast, AA. and Salz, HK. 2001. Nucleic Acids Res. 29: 3841-7. PMID: 11557816
- Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. | Jurica, MS., et al. 2002. RNA. 8: 426-39. PMID: 11991638
- Molecular cloning of the cDNA for the human U2 snRNA-specific A' protein. | Sillekens, PT., et al. 1989. Nucleic Acids Res. 17: 1893-906. PMID: 2928112
- Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. | Crispino, JD., et al. 1994. Science. 265: 1866-9. PMID: 8091213
- A coactivator of pre-mRNA splicing. | Blencowe, BJ., et al. 1998. Genes Dev. 12: 996-1009. PMID: 9531537
- Crystal structure of the spliceosomal U2B'-U2A' protein complex bound to a fragment of U2 small nuclear RNA. | Price, SR., et al. 1998. Nature. 394: 645-50. PMID: 9716128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
U2 SnRNP A Antibody (B-3) | sc-393804 | 200 µg/ml | $316.00 | |||
U2 SnRNP A Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-531763 | 200 µg Ab; 20 µg BP | $354.00 | |||
U2 SnRNP A Antibody (B-3) X | sc-393804 X | 200 µg/0.1 ml | $316.00 | |||
U2 SnRNP A Antibody (B-3) AC | sc-393804 AC | 500 µg/ml, 25% agarose | $416.00 | |||
U2 SnRNP A Antibody (B-3) HRP | sc-393804 HRP | 200 µg/ml | $316.00 | |||
U2 SnRNP A Antibody (B-3) FITC | sc-393804 FITC | 200 µg/ml | $330.00 | |||
U2 SnRNP A Antibody (B-3) PE | sc-393804 PE | 200 µg/ml | $343.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 488 | sc-393804 AF488 | 200 µg/ml | $357.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 546 | sc-393804 AF546 | 200 µg/ml | $357.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 594 | sc-393804 AF594 | 200 µg/ml | $357.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 647 | sc-393804 AF647 | 200 µg/ml | $357.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 680 | sc-393804 AF680 | 200 µg/ml | $357.00 | |||
U2 SnRNP A Antibody (B-3) Alexa Fluor® 790 | sc-393804 AF790 | 200 µg/ml | $357.00 |