U-937 nuclear extract: sc-2156

U-937 nuclear extract is derived from the U-937 cell line, a well established permanent human hematopoietic cell line. U-937 nuclear extract is utilized primarily in immunological research and provides a vital tool for investigating the nuclear mechanisms of monocyte/macrophage differentiation and function. This extract is particularly useful for studying the transcriptional regulation of genes involved in immune response and inflammation. Researchers employ U-937 nuclear extracts in a variety of assays, including electrophoretic mobility shift assays (EMSA) for DNA-binding activity, chromatin immunoprecipitation (ChIP) to study histone modifications and transcription factor binding, and co-immunoprecipitation experiments to identify and characterize protein-protein interactions within the nucleus. By analyzing these nuclear extracts, scientists gain insights into the regulatory mechanisms that control gene expression during immune activation and cellular stress response. The origin and quality of the U-937 cell line are carefully verified to ensure the reliability and reproducibility of experimental data, emphasizing the extract's role solely in fundamental research applications focused on understanding basic cellular processes and signaling pathways.

U-937 nuclear extract References:

1. VEGI, a new member of the TNF family activates nuclear factor-kappa B and c-Jun N-terminal kinase and modulates cell growth.  |  Haridas, V., et al. 1999. Oncogene. 18: 6496-504. PMID: 10597252
2. Analysis of the HIV-1 LTR NF-kappaB-proximal Sp site III: evidence for cell type-specific gene regulation and viral replication.  |  McAllister, JJ., et al. 2000. Virology. 274: 262-77. PMID: 10964770
3. Theophylline inhibits NF-kappaB activation in human peripheral blood mononuclear cells.  |  Umeda, M., et al. 2002. Int Arch Allergy Immunol. 128: 130-5. PMID: 12065913
4. Differential effects of catalase on apoptosis induction in human promonocytic cells. Relationships with heat-shock protein expression.  |  Sancho, P., et al. 2003. Mol Pharmacol. 63: 581-9. PMID: 12606765
5. C/EBP- and Tat-mediated activation of the HIV-1 LTR in CD34+ hematopoietic progenitor cells.  |  Quiterio, S., et al. 2003. Biomed Pharmacother. 57: 49-56. PMID: 12642037
6. Identification of a Vitamin D response element in the human insulin receptor gene promoter.  |  Maestro, B., et al. 2003. J Steroid Biochem Mol Biol. 84: 223-30. PMID: 12711007
7. Macrophage migration inhibitory factor promotes innate immune responses by suppressing glucocorticoid-induced expression of mitogen-activated protein kinase phosphatase-1.  |  Roger, T., et al. 2005. Eur J Immunol. 35: 3405-13. PMID: 16224818
8. Modulation of HSP70 and HSP27 gene expression by the differentiation inducer sodium butyrate in U-937 human promonocytic leukemia cells.  |  García-Bermejo, L., et al. 1995. Leuk Res. 19: 713-8. PMID: 7500647
9. The zinc finger transcription factor Egr-1 is essential for and restricts differentiation along the macrophage lineage.  |  Nguyen, HQ., et al. 1993. Cell. 72: 197-209. PMID: 7678779
10. CD18 (beta 2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity.  |  Rosmarin, AG., et al. 1995. Proc Natl Acad Sci U S A. 92: 801-5. PMID: 7846055
11. Vitamin D3- and retinoic acid-induced monocytic differentiation: interactions between the endogenous vitamin D3 receptor, retinoic acid receptors, and retinoid X receptors in U-937 cells.  |  Botling, J., et al. 1996. Cell Growth Differ. 7: 1239-49. PMID: 8877104
12. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus major immediate-early promoters in monocyte and T-lymphocyte cell types.  |  Chan, YJ., et al. 1996. J Virol. 70: 8590-605. PMID: 8970984
13. Mechanisms of resistance in a human cell line exposed to sequential topoisomerase poisoning.  |  Saleem, A., et al. 1997. Cancer Res. 57: 5100-6. PMID: 9371509