U-73122 (CAS 112648-68-7)

Usage :

U-73122 is only sparingly soluble in aqueous media. Because of this U-73122 can only be delivered to the cell by forming a complex with serum proteins or by dissolving it with an organic solvent.

Preparation of Working Solutions:

1. Dissolve U-73122 in Dichloromethane (sc-239703) or Chloroform (sc-239527).

2. Aliquot amount needed for a single experiemnt into suitable vials.

3. Evaporate the methylene chloride or chloroform using nitrogen.

4. The result is a film of U-73122 that can be dissolved in DMSO (5mM), ethanol (2mM in 100%) or an aqueous medium that contains serum or serum albumin (1-10 mg/mL), which forms a complex with U-73122, thus eliminating the need to use an organic solvent to deliver the compound to the cells.

Useful Range of Concentrations:

Useful concentrations of U-73122 range between 0.1 and 10 μM. Several factors affect the necessary concentration including: cell density, cell type, medium concentration and the particular variable that is being measured.

Physical State :

Solid

Solubility :

Soluble in anhydrous DMSO (1 mg/ml), DMF, PBS pH7.2, anhydrous Chloroform (3 mg/ml), anhydrous Ethanol (500 µg/ml), dichloromethane, and aqueous solutions (sparingly). Insoluble in water.

Storage :

Store at room temperature

Melting Point :

121-122° C

Boiling Point :

617.14° C at 760 mmHg (Predicted)

Density :

1.17 g/cm³ (Predicted)

Refractive Index :

n²⁰_D 1.59 (Predicted)

IC50 :

Recombinant enzyme: IC₅₀ = 30 nM; Neutrophil Elastase: IC₅₀ = 50 nM (human); Parathyroid hormone-induced Ca2+ transients: IC₅₀ = 0.5 µM; Platelet aggregation induced by collagen: IC₅₀ = 0.6 µM; ET-1 hormone-induced Ca2+ transients: IC₅₀ = 0.8 µM; PMNL homogenates: IC₅₀ = 2.4 µM; Platelet aggregation induced by thrombin : IC₅₀ = 5 µM; HIV-1 integrase: IC₅₀ = 7 µM

pK Values :

pK_b: 10.69 (Predicted)

PubChem CID :

SMILES :

CC12CCC3C(C1CCC2NCCCCCCN4C(=O)C=CC4=O)CCC5=C3C=CC(=C5)OC