Twinfilin-1 Antibody (E-4): sc-376539
- Twinfilin-1 Antibody (E-4) is a mouse monoclonal IgG2b κ Twinfilin-1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 175-236 mapping near the C-terminus of Twinfilin-1 of human origin
- Twinfilin-1 Antibody (E-4) is recommended for detection of Twinfilin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Twinfilin-1 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Twinfilin-1 Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Twinfilin-1 Antibody (E-4) (see ordering information below).
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Twinfilin-1 Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Twinfilin-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Twinfilin-1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Twinfilin-1 plays a crucial role in regulating cytoskeletal dynamics by binding to actin monomers, thereby preventing their assembly into filaments. This function is vital for maintaining cellular structure and facilitating processes such as cell motility and division. Twinfilin-1 is composed of two actin-depolymerizing factor homology domains, which allow coordination of filament severing and monomer sequestering at sites of rapid actin turnover. Twinfilin-1 is predominantly expressed in embryos and most adult non-muscle cell types, where binding to ADP-G-actin inhibits filament assembly by blocking nucleotide exchange on actin monomers. Additionally, Twinfilin-1 activity is modulated by phosphatidylinositol (4,5)-bisphosphate, which inhibits function, while small GTPases like Rac1 and Cdc42 promote redistribution to membrane ruffles and cell-cell contacts, highlighting a dynamic role in cellular signaling and structure.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Twinfilin-1 Antibody (E-4) References:
- Mouse A6/twinfilin is an actin monomer-binding protein that localizes to the regions of rapid actin dynamics. | Vartiainen, M., et al. 2000. Mol Cell Biol. 20: 1772-83. PMID: 10669753
- Interactions with PIP2, ADP-actin monomers, and capping protein regulate the activity and localization of yeast twinfilin. | Palmgren, S., et al. 2001. J Cell Biol. 155: 251-60. PMID: 11604420
- Twinfilin is required for actin-dependent developmental processes in Drosophila. | Wahlström, G., et al. 2001. J Cell Biol. 155: 787-96. PMID: 11724820
- Twinfilin, a molecular mailman for actin monomers. | Palmgren, S., et al. 2002. J Cell Sci. 115: 881-6. PMID: 11870207
- Mammals have two twinfilin isoforms whose subcellular localizations and tissue distributions are differentially regulated. | Vartiainen, MK., et al. 2003. J Biol Chem. 278: 34347-55. PMID: 12807912
- Biological role and structural mechanism of twinfilin-capping protein interaction. | Falck, S., et al. 2004. EMBO J. 23: 3010-9. PMID: 15282541
- Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. | Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
- Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo. | Moseley, JB., et al. 2006. J Cell Sci. 119: 1547-57. PMID: 16569665
- Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein. | Goode, BL., et al. 1998. J Cell Biol. 142: 723-33. PMID: 9700161
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Twinfilin-1 Antibody (E-4) | sc-376539 | 200 µg/ml | $322.00 | |||
Twinfilin-1 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-522998 | 200 µg Ab, 40 µg BP | $361.00 | |||
Twinfilin-1 Antibody (E-4): m-IgG2b BP-HRP Bundle | sc-549083 | 200 µg Ab; 10 µg BP | $361.00 | |||
Twinfilin-1 Antibody (E-4) AC | sc-376539 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Twinfilin-1 Antibody (E-4) HRP | sc-376539 HRP | 200 µg/ml | $322.00 | |||
Twinfilin-1 Antibody (E-4) FITC | sc-376539 FITC | 200 µg/ml | $336.00 | |||
Twinfilin-1 Antibody (E-4) PE | sc-376539 PE | 200 µg/ml | $349.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 488 | sc-376539 AF488 | 200 µg/ml | $364.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 546 | sc-376539 AF546 | 200 µg/ml | $364.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 594 | sc-376539 AF594 | 200 µg/ml | $364.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 647 | sc-376539 AF647 | 200 µg/ml | $364.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 680 | sc-376539 AF680 | 200 µg/ml | $364.00 | |||
Twinfilin-1 Antibody (E-4) Alexa Fluor® 790 | sc-376539 AF790 | 200 µg/ml | $364.00 |