TTI1 Antibody (B-1): sc-365119

TTI1 Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects TTI1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TTI1 (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TTI1, also known as TELO2-interacting protein 1 homolog or SMG10, is a crucial 1,089 amino acid protein that plays a significant role in regulating DNA damage response (DDR) and is a key component of the TTT complex, which is essential for stabilizing protein levels of the phosphatidylinositol 3-kinase (PIKK) family. The TTT complex is vital for cellular resistance to DNA damage caused by various stresses, including ionizing radiation, ultraviolet light, and mitomycin C. TTI1 is involved in assembling and stabilizing mTORC1 and mTORC2 complexes, which are critical for cell growth and metabolism. TTI1 is primarily located in the cytoplasm and nucleus, where TTI1 interacts with other proteins to facilitate proper cellular responses to stress. Additionally, TTI1 undergoes post-translational modifications, including phosphorylation at serine 459, which can influence function and interactions. The gene encoding TTI1 is located on human chromosome 20, highlighting evolutionary conservation and importance across species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TTI1 Antibody (B-1) References:

1. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
2. Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.  |  Daub, H., et al. 2008. Mol Cell. 31: 438-48. PMID: 18691976
3. AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.  |  Izumi, N., et al. 2010. Sci Signal. 3: ra27. PMID: 20371770
4. Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly.  |  Kaizuka, T., et al. 2010. J Biol Chem. 285: 20109-16. PMID: 20427287
5. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.  |  Takai, H., et al. 2010. Genes Dev. 24: 2019-30. PMID: 20801936
6. A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.  |  Hurov, KE., et al. 2010. Genes Dev. 24: 1939-50. PMID: 20810650
7. Initial characterization of the human central proteome.  |  Burkard, TR., et al. 2011. BMC Syst Biol. 5: 17. PMID: 21269460
8. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro.  |  Ishikawa, K., et al. 1997. DNA Res. 4: 307-13. PMID: 9455477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TTI1 Antibody (B-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TTI1 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TTI1 Antibody (B-1) Specifications

Alternate Product Name: TTI1

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: TTI1

Epitope Species: human

Product Reactivity/Detection: TTI1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TTI1 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin