TSSC4 Double Nickase Plasmid (h): sc-410490-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSSC4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TSSC4 Double Nickase Plasmid (h) and TSSC4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TSSC4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSSC4 Double Nickase Plasmid (h) | sc-410490-NIC | 20 µg | $410.00 | |||
TSSC4 Double Nickase Plasmid (h2) | sc-410490-NIC-2 | 20 µg | $410.00 |
TSSC4 (tumor suppressing subtransferable candidate 4) is a human protein-coding gene originally mapped to the imprinted 11p15.5 region and studied in the context of growth control and tumor biology. Although its molecular function remains incompletely defined, TSSC4 has been linked to cellular programs that govern proliferation and survival, and its genomic neighborhood is frequently examined for epigenetic regulation and allele-specific expression. Dysregulation within the 11p15.5 locus is associated with developmental growth disorders and oncogenic processes, making TSSC4 a useful target for mechanistic studies of imprinting-associated pathways. Experimental perturbation of TSSC4 supports investigation of how locus-level regulation influences cell-cycle behavior and stress-responsive transcriptional networks.
TSSC4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TSSC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TSSC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TSSC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TSSC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.