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p-Troponin I-C Antibody (5E6): sc-52274

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Datasheets
  • p-Troponin I-C Antibody (5E6) is a mouse monoclonal IgG2b p-Troponin I-C antibody provided at 100 µg/ml
  • raised against phosphorylated form of human cardiac troponin I
  • recommended for detection of a phosphorylated form of cardiac Troponin I of mouse, rat and human origin by WB and IP
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Troponin I-C Antibody (5E6). This work is in progress.
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    p-Troponin I-C Antibody (5E6) is a mouse monoclonal IgG2b antibody that detects p-Troponin I-C protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). anti-p-Troponin I-C antibody (5E6) is available as the non-conjugated format. p-Troponin I-C protein plays a critical role in cardiac muscle contraction by regulating the interaction between actin and myosin, which are essential for muscle contraction and movement. p-Troponin I-C is part of the troponin complex, which consists of three subunits: Troponin C, Troponin I, and Troponin T. Troponin C binds calcium ions, initiating a conformational change that allows myosin to interact with actin filaments, thereby facilitating muscle contraction. p-Troponin I-C is particularly important in cardiac health, as elevated levels in the bloodstream can serve as a biomarker for myocardial injury or stress. p-Troponin I-C antibody (5E6) enables valuable insights into cardiac function and underlying mechanisms of heart disease, making this reagent essential for both basic and clinical research applications.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    p-Troponin I-C Antibody (5E6)

    sc-52274
    100 µg/ml
    $322.00

    I am using p-Troponin I-C (5E6): sc-52274 antibody for immunoprecipitation, how much antibody should I use?

    Asked by: DefinitelyNotMatt
    Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Strong signal observed in WB in rat heartStrong signal observed in WB in rat heart and mouse heart tissue extracts. -SCBT QC
    Date published: 2014-06-13
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    p-Troponin I-C Antibody (5E6) is rated 4.0 out of 5 by 1.
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