Troponin I Antibody (E-9): sc-365446
- Troponin I Antibody (E-9) is a mouse monoclonal IgM κ Troponin I antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 186-209 at the C-terminus of Troponin I-C of human origin
- recommended for detection of Troponin I, cardiac muscle of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with Troponin I, slow skeletal muscle and Troponin I, fast skeletal muscle
- available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Troponin I (E-9): sc-365446.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Troponin I Antibody (E-9). This work is in progress.
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Troponin I Antibody (E-9) is a mouse monoclonal IgM kappa light chain antibody designed to detect Troponin I, also known as cardiac troponin I (cTnI), slow skeletal troponin I, and fast skeletal troponin I, across mouse, rat, and human species. Anti-Troponin I antibody (E-9) targets a precise epitope mapping between amino acids 186-209 at the C-terminus of human Troponin I-C, ensuring accurate and reliable binding for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Troponin I plays a crucial role in the regulation of muscle contraction by inhibiting the actin-myosin interaction in the absence of calcium ions, thereby serving as a key regulatory component within the troponin complex, which also includes Troponin T (cTnT) and Troponin C (cTnC). The function of Troponin I is essential for proper cardiac muscle contraction and relaxation, making Troponin I a vital biomarker for cardiac injury and related diseases such as myocardial infarction and heart failure. Anti-Troponin I antibody (E-9) allows researchers to study Troponin I interactions with other proteins like actin, myosin, and other troponin subunits, which are critical for maintaining muscle function and responding to physiological stimuli. Additionally, Troponin I′s interactions with regulatory proteins such as tropomyosin highlight Troponin I′s importance in the contractile apparatus of muscle cells. Troponin I monoclonal antibody (E-9) recognition across multiple species facilitates comparative studies and translational research, enhancing our understanding of muscle physiology and pathology. Anti-Troponin I antibody (E-9) is an indispensable tool for researchers investigating muscle contraction mechanisms, cardiac biomarkers, and related pathological conditions, providing high specificity and sensitivity in detecting and analyzing Troponin I within various experimental contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
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Troponin I Antibody (E-9) References:
- Structure and expression of the murine slow/cardiac troponin C gene. | Parmacek, MS. and Leiden, JM. 1989. J Biol Chem. 264: 13217-25. PMID: 2753913
- cDNA clone and expression analysis of rodent fast and slow skeletal muscle troponin I mRNAs. | Koppe, RI., et al. 1989. J Biol Chem. 264: 14327-33. PMID: 2760067
- A direct regulatory role for troponin T and a dual role for troponin C in the Ca2+ regulation of muscle contraction. | Potter, JD., et al. 1995. J Biol Chem. 270: 2557-62. PMID: 7852318
- Structure and regulation of the mouse cardiac troponin I gene. | Ausoni, S., et al. 1994. J Biol Chem. 269: 339-46. PMID: 8276817
- Actin cytoskeleton. Setting the pace of cell movement. | Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
- Myosins: matching functions with motors. | Baker, JP. and Titus, MA. 1998. Curr Opin Cell Biol. 10: 80-6. PMID: 9484598
- A new look at thin filament regulation in vertebrate skeletal muscle. | Squire, JM. and Morris, EP. 1998. FASEB J. 12: 761-71. PMID: 9657517
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Troponin I Antibody (E-9) | sc-365446 | 200 µg/ml | $316.00 | |||
Troponin I Antibody (E-9) AC | sc-365446 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Troponin I Antibody (E-9) Alexa Fluor® 488 | sc-365446 AF488 | 200 µg/ml | $357.00 | |||
Troponin I Antibody (E-9) Alexa Fluor® 647 | sc-365446 AF647 | 200 µg/ml | $357.00 | |||
Troponin I Antibody (E-9) FITC | sc-365446 FITC | 200 µg/ml | $330.00 | |||
Troponin I Antibody (E-9) HRP | sc-365446 HRP | 200 µg/ml | $316.00 | |||
Troponin I (E-9) Neutralizing Peptide | sc-365446 P | 100 µg/0.5 ml | $68.00 | |||
Troponin I Antibody (E-9) PE | sc-365446 PE | 200 µg/ml | $343.00 |