Tropomyosin Antibody (F-6): sc-74480

Tropomyosin Antibody (F-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Tropomyosin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Tropomyosin antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tropomyosin is a crucial structural protein found in nearly all eukaryotic cells, where Tropomyosin plays a vital role in muscle contraction and cellular motility by binding to actin filaments. Tropomyosin is essential for stabilizing actin filament structure and regulating interaction between actin and myosin, which is fundamental for muscle contraction. Tropomyosin α, encoded by TPM1 gene located on human chromosome 15q22.1, undergoes alternative splicing to produce several isoforms, including those specific to skeletal and smooth muscle, as well as fibroblasts. Similarly, Tropomyosin β, encoded by TPM2 gene on chromosome 9p13.3, also generates multiple isoforms through alternative splicing. Interaction of Tropomyosin with other proteins, such as troponin I and caldesmon, further highlights Tropomyosin′s importance in muscle function and regulation, as Tropomyosin enhances rigidity of actin filaments and modulates smooth muscle contraction. This multifaceted role of Tropomyosin underscores Tropomyosin′s significance in both muscle physiology and various cellular processes, making Tropomyosin (F-6) antibody an invaluable tool for researchers studying muscle biology and related disorders.

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Tropomyosin Antibody (F-6) References:

1. Tropomyosin and actin isoforms modulate the localization of tropomyosin strands on actin filaments.  |  Lehman, W., et al. 2000. J Mol Biol. 302: 593-606. PMID: 10986121
2. Binding of tropomyosin-troponin to actin increases filament bending stiffness.  |  Goldmann, WH. 2000. Biochem Biophys Res Commun. 276: 1225-8. PMID: 11027614
3. Periodic binding of troponin C.I and troponin I to tropomyosin-actin filaments.  |  Ohtsuki, I. and Shiraishi, F. 2002. J Biochem. 131: 739-43. PMID: 11983082
4. Tropomyosin and the steric mechanism of muscle regulation.  |  Lehman, W. and Craig, R. 2008. Adv Exp Med Biol. 644: 95-109. PMID: 19209816
5. Tropomyosin-binding properties modulate competition between tropomodulin isoforms.  |  Colpan, M., et al. 2016. Arch Biochem Biophys. 600: 23-32. PMID: 27091317
6. Binding of S100A6 to actin and the actin-tropomyosin complex.  |  Jurewicz, E., et al. 2020. Sci Rep. 10: 12824. PMID: 32733033
7. Tropomyosin autoantibodies associated with checkpoint inhibitor myositis.  |  Zaenker, P., et al. 2020. Oncoimmunology. 9: 1804703. PMID: 32923166
8. Novel Mutation Glu98Lys in Cardiac Tropomyosin Alters Its Structure and Impairs Myocardial Relaxation.  |  Matyushenko, AM., et al. 2023. Int J Mol Sci. 24: PMID: 37569730
9. Sarcomeric tropomyosin expression during human iPSC differentiation into cardiomyocytes.  |  Dube, DK., et al. 2024. Cytoskeleton (Hoboken). 81: 448-472. PMID: 38470291
10. Myosin-1C differentially displaces tropomyosin isoforms altering their inhibition of motility.  |  Pollard, LW., et al. 2024. J Biol Chem. 300: 107539. PMID: 38971309
11. Fine mapping of five human skeletal muscle genes: alpha-tropomyosin, beta-tropomyosin, troponin-I slow-twitch, troponin-I fast-twitch, and troponin-C fast.  |  Tiso, N., et al. 1997. Biochem Biophys Res Commun. 230: 347-50. PMID: 9016781
12. Actin and tropomyosin isoforms in morphogenesis.  |  Gunning, P., et al. 1997. Anat Embryol (Berl). 195: 311-5. PMID: 9108196

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tropomyosin Antibody (F-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Tropomyosin Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tropomyosin Antibody (F-6) Specifications

Alternate Product Name: TPM1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-284

Epitope Target: Tropomyosin α1

Epitope Species: human

Product Reactivity/Detection: Tropomyosin α1-4 and β1-3 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Tropomyosin Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Tropomyosin α antibody is Tropomyosin α Antibody (F-6): sc-376541.