TROP-2 Antibody (B-9) recommended for detection of TROP-2 of human origin by WB, IP, IF, IHC(P) and ELISA

TROP-2 Antibody (B-9): sc-376746

TROP-2 Antibody (B-9) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-TROP-2 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) TROP-2 antibody provided at 200 µg/ml
    • raised against amino acids 141-225 mapping within an internal region of TROP-2 of human origin
    • TROP-2 Antibody (B-9) is recommended for detection of TROP-2 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TROP-2 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TROP-2 (B-9): sc-376746.
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Can TROP-2 (B-9): sc-376746 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TROP-2 (B-9): sc-376746 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong Staining Excellent strength of stain seen when used in IHC with FFPE human tissue samples.
Date published: 2017-01-23
Rated 5 out of 5 by from Great Western Blot antibody We have Trop2 knock-out and overexpression stable cell lines. The Western blot staining show pretty good specificity and sensitivity. Single band and no non-specific binding.
Date published: 2016-10-26
Rated 5 out of 5 by from Produced exceptional immunoperoxidase membrane Produced exceptional immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Good Western Blot data of TROP Good Western Blot data of TROP-2 expression in non-transfected and human TROP-2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-04-11
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