TRM61 Double Nickase Plasmid (h): sc-408677-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRM61 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRM61 Double Nickase Plasmid (h) and TRM61 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRMT61A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRM61 Antibody (C145I165): sc-81062
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRM61 Double Nickase Plasmid (h) | sc-408677-NIC | 20 µg | $410.00 | |||
TRM61 Double Nickase Plasmid (h2) | sc-408677-NIC-2 | 20 µg | $410.00 |
Human TRMT61A encodes TRM61, a catalytic subunit of the tRNA m1A58 methyltransferase complex that installs N1-methyladenosine at position 58 in initiator and elongator tRNAs. This modification supports tRNA structural stability, proper aminoacylation, and accurate translation, linking TRM61 activity to RNA processing, proteostasis, and cellular stress responses. Perturbation of tRNA modification pathways can disrupt global translational control and has been associated with growth defects and altered sensitivity to metabolic and proteotoxic stress in model systems. TRMT61A is therefore studied in the context of tRNA epitranscriptomic regulation, ribosome-associated quality control, and disease-relevant mechanisms tied to dysregulated protein synthesis.
TRM61 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRMT61A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRMT61A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRMT61A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRMT61A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.