Tris succinate (CAS 85169-32-0)

Tris succinate is a specialized variant of the widely used biological buffer Tris (tris(hydroxymethyl)aminomethane). This compound integrates the buffering capacity of Tris with the succinate moiety, enhancing its utility in various biochemical and molecular biology research applications. The succinate group, a key intermediate in the citric acid cycle, imparts additional properties that make Tris succinate particularly suitable for experiments requiring precise pH control within the physiological range (approximately pH 7.0-9.0). The mechanism of action of Tris succinate as a buffer lies in its ability to undergo reversible protonation and deprotonation, effectively stabilizing the pH of a solution even when acids or bases are added. This characteristic is for maintaining the stability of biological molecules and ensuring the accuracy of enzymatic reactions, which are highly sensitive to pH fluctuations. In research applications, Tris succinate is employed in a variety of protocols, including those involving nucleic acid extraction and purification, protein stabilization, and gel electrophoresis, where its buffering capabilities are indispensable for achieving reliable results.