Stock solution. Routinely used in the affinity purification of antibodies via Protein A agarose (sc-2001). In Protein A-agarose antibody purifications, the 1M stock solution is added to sera at 1/10 of total sera volume in order to optimize binding of antibody to column. In addition, 100 mM and 10 mM Tris solutions are used to wash Protein A column prior to elution.
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