Tris phosphate monobasic (CAS 6992-39-8)

Tris phosphate monobasic, chemically known as tris(hydroxymethyl)aminomethane phosphate, is a derivative of the widely used Tris buffer, modified by the addition of a phosphate group. This compound combines the buffering capabilities of Tris with the phosphate group′s properties, making it particularly useful in molecular biology and biochemistry research. With a CAS number of 6992-39-8, Tris phosphate monobasic serves as a buffering agent that can effectively maintain pH in the range typically around 7.0 to 9.0, which is crucial for most biological reactions. The mechanism of action involves the phosphate group accepting or donating protons in response to changes in pH, thus stabilizing the environmental pH and preventing drastic shifts that could potentially disrupt biochemical processes. In research, Tris phosphate monobasic is often used in buffer solutions for DNA and RNA extraction protocols, where maintaining a stable and precise pH is necessary for the efficient lysis of cells and subsequent stabilization of nucleic acids. Additionally, its use extends to protein purification techniques and gel electrophoresis, where the buffer′s ability to maintain pH without interacting with or denaturing the proteins is crucial. This makes Tris phosphate monobasic a versatile and valuable tool in the preparation of samples and execution of experiments where pH stability is paramount.