Tris phosphate dibasic (CAS 108321-11-5)

Tris phosphate dibasic, a derivative of the widely used Tris buffer, is a chemical compound that serves as an effective buffering agent in various biochemical and molecular biology applications. Unlike its parent compound, Tris (tris(hydroxymethyl)aminomethane), Tris phosphate dibasic provides unique buffering capabilities, particularly useful in the pH range of 7.0 to 9.0. This buffer compound functions by stabilizing the pH through its phosphate groups, which can donate or accept protons in response to changes in the hydrogen ion concentration of the solution. This dynamic equilibrium is vital for maintaining a consistent pH, especially in environments where biochemical reactions are highly sensitive to even minor pH fluctuations. In research, Tris phosphate dibasic is often employed in the preparation of buffer solutions for DNA, RNA, and protein isolation procedures, where maintaining an optimal and stable pH is crucial for the integrity and functionality of the macromolecules. Furthermore, its use extends to electrophoresis applications, particularly in the running and transfer buffers where precise pH control is essential for the accurate migration and transfer of nucleic acids and proteins. The buffer′s robustness at higher pH levels makes it particularly suitable for experiments involving enzymatic reactions that prefer or require an alkaline environment.