Tris hydrochloride solution, pH 7.4 CAS: 1185-53-1 (solid)
MW: 157.60
Non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers.

Tris hydrochloride solution, pH 7.4 (CAS 1185-53-1 (solid))

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Synonym: Tris(hydroxymethyl)aminomethane hydrochloride solution, pH 7.4
Application: Non-sterile; 0.2 µm filtered, 1M stock solution used to make buffers
CAS Number: 1185-53-1 (solid)
Molecular Weight: 157.60
Molecular Formula: NH2C(CH2OH)3·HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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pH 7.4, 1 M. Tris hydrochloride has been used as a component of extraction buffer e.g. phenol extraction of DNA or RNA; and as a component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE. The pH values of Tris buffer solutions are temperature and concentration-dependent. Between 5 °C and 25 °C, the pH value increases an average of 0.03 pH units for each °C decrease in temperature. As the buffer temperature increases from 25 °C to 37 °C, the pH value decreases an average of 0.025 pH units per °C. Increasing the concentration of Tris from 0.05 M to 0.5M will increase the pH value by about 0.05 units. Decreasing the concentration from 0.05 M to 0.005 M will decrease the pH value by about 0.05 units.

Formulation :
1 M
Physical State :
pH :
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :

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The pH of Tris-HCL in water alone is much lower than 7.4, do you adjust up by using the Tris base (CAS 77-86-1) or some other base?

Asked by: CEADOG
Thank you for your question. The pH is adjusted up using 5 M NaOH.
Answered by: Technical Support
Date published: 2019-09-11
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