TRIS Glycine buffer solution, also called Running buffer and similar to Towbin buffer, is supplied as 1L of 10X concentrate that can be diluted to a 1X solution containing 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3. Researchers may check and adjust the final pH using a sensitive pH meter.
TG-SDS Buffer, 10X Powder Pack (sc-296527)
1. Towbin, H., et al. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354. PMID: 388439<br>
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