Tris citrate dibasic solution (CAS 108321-34-2)

Tris citrate dibasic solution, a compound containing tris(hydroxymethyl)aminomethane (Tris) and citric acid, is a crucial reagent in various scientific research fields, notably in molecular biology, biochemistry, and analytical chemistry. This solution serves as a buffer system, providing a stable pH environment for biochemical and enzymatic reactions. Tris acts as a buffering agent, maintaining the desired pH level, while citrate ions contribute to the buffer capacity and stability of the solution. This buffering capacity is essential for maintaining optimal conditions in a wide range of experimental procedures, ensuring accurate and reproducible results. Moreover, Tris citrate dibasic solution finds applications in nucleic acid electrophoresis, particularly in agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE). It is utilized as a running buffer, facilitating the migration of nucleic acids through the gel matrix and enabling their separation based on size or charge. Additionally, this solution is employed in the preparation of nucleic acid staining and visualization solutions used in gel-based assays. It helps maintain the stability and integrity of nucleic acids during staining and imaging procedures, ensuring accurate detection and quantification of DNA or RNA bands. Furthermore, Tris citrate dibasic solution may be utilized in other biochemical and molecular biology applications, including protein purification, enzyme assays, and cell culture experiments, where precise pH control is essential for optimal experimental outcomes.