Reconstitute powder in ddH20 and empty contents of Tween-20 vial (rinse vial thoroughly) into liquid, bringing final volume to 100 ml (10X). Filter solution through a 0.22 μm filter into a sterile flask. Makes 1 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. Final (1X) pH 7.4 ±0.2 (may need slight pH adjustment).
外观 :
Powder
物理状态 :
Solid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.