Date published: 2025-9-17

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Tris Buffered Saline with Tween 20: 500 ml of 10X

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Alternate Names:
10X TBST
Application:
Tris Buffered Saline with Tween 20: 500 ml of 10X is a convenient prepared buffer for use in various applications
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Tris Buffered Saline with Tween 20: 500 ml of 10X is a high-quality 10X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)

Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)


Tris Buffered Saline with Tween 20: 500 ml of 10X References

  1. STAT3 activation abrogates growth factor dependence and contributes to head and neck squamous cell carcinoma tumor growth in vivo.  |  Kijima, T., et al. 2002. Cell Growth Differ. 13: 355-62. PMID: 12193474
  2. Ginsenoside Rb1 ameliorates liver fat accumulation by upregulating perilipin expression in adipose tissue of db/db obese mice.  |  Yu, X., et al. 2015. J Ginseng Res. 39: 199-205. PMID: 26199550
  3. Muscadine Grape (Vitis rotundifolia) or Wine Phytochemicals Reduce Intestinal Inflammation in Mice with Dextran Sulfate Sodium-Induced Colitis.  |  Li, R., et al. 2017. J Agric Food Chem. 65: 769-776. PMID: 28112913
  4. Spexin Modulates Functions of Rat Endocrine Pancreatic Cells.  |  Sassek, M., et al. 2018. Pancreas. 47: 904-909. PMID: 29912854
  5. Gold nanoparticles attenuates bacterial sepsis in cecal ligation and puncture mouse model through the induction of M2 macrophage polarization.  |  Taratummarat, S., et al. 2018. BMC Microbiol. 18: 85. PMID: 30119646
  6. Shank3 regulates striatal synaptic abundance of Cyld, a deubiquitinase specific for Lys63-linked polyubiquitin chains.  |  Jin, C., et al. 2019. J Neurochem. 150: 776-786. PMID: 31215654
  7. Elevated TRIM23 expression predicts cisplatin resistance in lung adenocarcinoma.  |  Zhang, Y., et al. 2020. Cancer Sci. 111: 637-646. PMID: 31677335
  8. High-fat Feeding Causes Inflammation and Insulin Resistance in the Ventral Tegmental Area in Mice.  |  Mizoguchi, A., et al. 2021. Neuroscience. 461: 72-79. PMID: 33609637
  9. Increased substance P and synaptic remodeling occur in the trigeminal sensory system with sustained osteoarthritic temporomandibular joint sensitivity.  |  Sperry, MM., et al. 2021. Pain Rep. 6: e911. PMID: 33977183
  10. Attenuation of hepatic fibrosis by p-Coumaric acid via modulation of NLRP3 inflammasome activation in C57BL/6 mice.  |  Truong, TMT., et al. 2023. J Nutr Biochem. 112: 109204. PMID: 36400112

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Tris Buffered Saline with Tween 20: 500 ml of 10X, 500 ml

sc-362310
500 ml
$40.00