Tris Buffered Saline with Tween 20: 1 L of 10X A convenient prepared buffer for use in various applications.

Tris Buffered Saline with Tween 20: 1 L of 10X

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Alternate Names: Tris Buffered Saline with Tween 20: 1 L of 10X also known as 10X TBST
Application: Tris Buffered Saline with Tween 20: 1 L of 10X is a convenient prepared buffer for use in various applications
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

Tris Buffered Saline with Tween 20: 1 L of 10X is a high-quality 10X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)

Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)


References

1. Thomson, BR, et al. Methods Mol Biol. 2019. 1846: 153-160. PMID: 30242758
2. Doran, TM, et al. Bioorg Med Chem Lett. 2016. 25(21): 4910-4917. PMID: 26067174
3. Kemper, C. et al. Bio Protoc. 2014. 4(16):e1205. PMID: 29094056

Usage :
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 10 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
Formulation :
10X
Physical State :
Liquid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Tris Buffered Saline with Tween 20: 1 L of 10X  Product Citations

See how others have used Tris Buffered Saline with Tween 20: 1 L of 10X. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 16 total

PMID: # 27009863  Bhadury, J. et al. 2016. Oncotarget.

PMID: # 27150226  Kondaurova, EM. et al. 2016. Behavioural brain research. 310: 20-5.

PMID: # 26893688  Shi, J. et al. 2016. Oncology letters. 11: 1043-1050.

PMID: # 26622777  Seven, D. et al. 2015. Oncology letters. 10: 1927-1931.

PMID: # 25815442  Lee, C. et al. 2015. Mol Med Rep. 12: 1485-92.

PMID: # 25312823  Liu, SS. et al. 2014. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 68: 1031-6.

PMID: # 25270520  Minn, YK. et al. 2014. International journal of oncology. 45: 2403-10.

PMID: # 24713807  Wagner, JM. et al. 2014. PloS one. 9: e94401.

PMID: # 23224340  Shoae-Hassani, A. et al. 2013. Archivum immunologiae et therapiae experimentalis. 61: 75-83.

PMID: # 23110163  Fallica, B. et al. 2012. PloS one. 7: e48024.

Citations 1 to 10 of 16 total

Hello, Is it necessary to use a filter? can I just dilute the 10X solution by adding 10 ml of the conc. TBST to 900 of water?

Asked by: Oceana
Thank you for your question. Dilute to a 1X solution with ddH20. Filter solution through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X TBST buffer. Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%. You can find more information here: https://www.scbt.com/scbt/product/tris-buffered-saline-with-tween-20-1-l-of-10x If you have any further questions, please contact Technical Service.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-11
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