Date published: 2025-10-19

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Tris Buffered Saline: 5 L of 20X

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Alternate Names:
20X TBS
Application:
Tris Buffered Saline: 5 L of 20X is a convenient prepared buffer for use in various applications
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Tris Buffered Saline: 5 L of 20X is ready to use for dilution to a 1X working solution (pH 7.4). Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. It is an optimal formulation of salts and pH stabilizers which enable washing without disruption of antibody-antigen binding interactions.

Additional sizes and formats available:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)

Also available with Tween 20:
Tris Buffered Saline with Tween 20:1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20:100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20:500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20:1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)


Tris Buffered Saline: 5 L of 20X References

  1. Embolization of portal vein branches induces hepatocyte replication in swine: a potential step in hepatic gene therapy.  |  Duncan, JR., et al. 1999. Radiology. 210: 467-77. PMID: 10207431
  2. Improvement of western blot test specificity for detecting equine serum antibodies to Sarcocystis neurona.  |  Rossano, MG., et al. 2000. J Vet Diagn Invest. 12: 28-32. PMID: 10690772
  3. Placental expression of syndecan 1 is diminished in preeclampsia.  |  Jokimaa, VI., et al. 2000. Am J Obstet Gynecol. 183: 1495-8. PMID: 11120517
  4. Expression of RANKL in osteolytic membranes: association with fibroblastic cell markers.  |  Ramage, SC., et al. 2007. J Bone Joint Surg Am. 89: 841-8. PMID: 17403809
  5. Chemical and morphological changes on platinum microelectrode surfaces in AC and DC fields with biological buffer solutions.  |  Gencoglu, A. and Minerick, A. 2009. Lab Chip. 9: 1866-73. PMID: 19532961
  6. Correlation between expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinase-2 and cervical lymph node metastasis of nasopharyngeal carcinoma.  |  Huang, T., et al. 2013. Ann Otol Rhinol Laryngol. 122: 210-5. PMID: 23577575
  7. Avidin-biotin amplification procedures for the detection of human terminal deoxynucleotidyl transferase in cell smears.  |  Fetterhoff, TJ. and McCarthy, RC. 1985. Am J Clin Pathol. 83: 565-70. PMID: 3887892
  8. A simple and rapid dot-immunobinding assay for zein and other prolamins.  |  Esen, A., et al. 1983. Anal Biochem. 132: 462-7. PMID: 6625179
  9. Downward blotting of proteins in a model based on apolipoprotein(a) phenotyping.  |  Nagy, B., et al. 1995. Anal Biochem. 231: 40-5. PMID: 8678318
  10. An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative.  |  Evers, P. and Uylings, HB. 1997. J Neurosci Methods. 72: 197-207. PMID: 9133585

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Tris Buffered Saline: 5 L of 20X, 5 L

sc-362305
5 L
$131.00

How is the formulation? (concentration NaCl and TRIS?)

Asked by: ivan c
Please see our SDS for composition information.
Answered by: Technical Support
Date published: 2018-12-20

For how long does the 20 x concentrate remains stable at room temperature?

Asked by: SCM4
Unopened Tris Buffered Saline: 20X (TBS 20X) is stable 1 year at RT. TBS solutions that become turbid should be discarded.
Answered by: Tech Service 11
Date published: 2017-03-17
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Rated 5 out of 5 by from Various publications cite Tris Buffered SalineVarious publications cite Tris Buffered Saline as a gentle wash solution (without Tween 20) for SDS-PAGE; enables washing of western blot membranes without disturbing antibody-antigen binding interactions. -SCBT Publication Review
Date published: 2015-03-17
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Tris Buffered Saline: 5 L of 20X is rated 5.0 out of 5 by 1.
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