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Tris base (CAS 77-86-1) - chemical structure image
Molecular structure of Tris base, CAS Number: 77-86-1
Tris base (CAS 77-86-1) - chemical structure image
Molecular structure of Tris base, CAS Number: 77-86-1
Molecular structure of Tris base, CAS Number: 77-86-1

Tris base (CAS 77-86-1)

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Alternate Names:
Tris base is also known as Tris(hydroxymethyl)aminomethane.
Application:
Tris base is widely used as a biological buffer or as a component of buffer formulations. Tris base has buffering capabilities in the pH range of 7.0-9.0.
CAS Number:
77-86-1
Purity:
≥99%
Molecular Weight:
121.14
Molecular Formula:
C4H11NO3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Description
Technical Information
Safety Information
SDS & Certificate of Analysis

Tris base is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. It has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris base has been reported to interfere with the activity of a number of enzymes, therefore it should be used carefully when studying proteins. Tris base is also known as THAM, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, and Tris(hydroxymethyl)aminomethane.


Tris base (CAS 77-86-1) References

  1. The treatment of acidosis in acute lung injury with tris-hydroxymethyl aminomethane (THAM).  |  Kallet, RH., et al. 2000. Am J Respir Crit Care Med. 161: 1149-53. PMID: 10764304
  2. The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition.  |  Desmarais, WT., et al. 2002. Structure. 10: 1063-72. PMID: 12176384
  3. Binding of Tris to Bacillus licheniformis alpha-amylase can affect its starch hydrolysis activity.  |  Ghalanbor, Z., et al. 2008. Protein Pept Lett. 15: 212-4. PMID: 18289113
  4. Conformational Dynamics of DNA G-Quadruplex in Solution Studied by Kinetic Capillary Electrophoresis Coupled On-line with Mass Spectrometry.  |  Mironov, GG., et al. 2014. ChemistryOpen. 3: 58-64. PMID: 24808992
  5. Effect of TRIS and bicarbonate as buffers on anesthetic efficacy of tricaine methane sulfonate in Zebrafish (Danio rerio).  |  Spears, J., et al. 2014. Zebrafish. 11: 590-6. PMID: 25264822
  6. In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver.  |  Ding, W., et al. 2016. J Vis Exp.. PMID: 27166647
  7. Mitigating the Effects of Electrode Biofouling-Induced Impedance for Improved Long-Term Electrochemical Measurements In Vivo.  |  Seaton, BT., et al. 2020. Anal Chem. 92: 6334-6340. PMID: 32298105
  8. Investigation of Break-Induced Replication in Yeast.  |  Osia, B., et al. 2021. Methods Mol Biol. 2153: 307-328. PMID: 32840789
  9. FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration.  |  Naeimipour, S., et al. 2020. Iran J Pharm Res. 19: 175-181. PMID: 33224222
  10. An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA.  |  He, L., et al. 2021. STAR Protoc. 2: 100504. PMID: 33997818
  11. Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells.  |  Holliday, H., et al. 2021. STAR Protoc. 2: 100514. PMID: 34013210
  12. Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation.  |  Nair, JD., et al. 2021. STAR Protoc. 2: 100992. PMID: 34934960
  13. A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii.  |  Elman, T. and Yacoby, I. 2022. STAR Protoc. 3: 101640. PMID: 36042878
  14. Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy.  |  Penumutchu, S., et al. 2022. STAR Protoc. 3: 101842. PMID: 36595882
  15. A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection.  |  Gong, XY. and Zhang, YB. 2022. STAR Protoc. 3: 101844. PMID: 36595883

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Tris base, 500 g

sc-3715
500 g
$33.00

Tris base, 1 kg

sc-3715A
1 kg
$58.00

Tris base, 5 kg

sc-3715B
5 kg
$251.00

Tris base, 10 kg

sc-3715C
10 kg
$556.00

What is the chemical purity of Trisbase or tromethamine by HPLC or CAD or any other instrumental methods except titration method?

Asked by: VinayKumar
Thank you for your question. This product has a specified purity of >99.5 %, but exact purity is lot dependent. Please contact our customer service team for a certificate of analysis of the most recent batch.
Answered by: Tech Support Europe
Date published: 2022-11-30

je voudrais consulter le fiche de données de sécurité de TRIS

Asked by: MN23
Merci pour votre question. La MSDS est disponible à télécharger directement en ligne sur la page web du produit, dans la partie "SDS et Certificat d'Analyse". Veuillez contacter notre Service Technique pour toute information complémentaire sur ce produit. Thank you for your question. You can download the MSDS for this product on its product page under 'SDS & Certificate of Analysis'. MSDS documentation for other articles from our ChemCruz product line can be downloaded on the individual product pages. If you have further questions, please contact our technical service directly.
Answered by: Technical Support Europe
Date published: 2019-02-19

interested in a price offer for: Tris base, 500 g sc-3715 500 g, CAS: 77-86-1

Asked by: Adva
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times.
Answered by: Technical Support
Date published: 2019-01-15

do you sell TrisBase-HCL? How much does it cost? Thanks

Asked by: LENny
Thank you for your question. Yes, we offer several different TrisBase-HCL. You can find them here: https://www.scbt.com/scbt/search?Ntt=Tris+base+hcl
Answered by: Tech Service
Date published: 2019-01-14

What is the country of origin?

Asked by: SCM4
Previous lots have originated in China.
Answered by: Tech Service 11
Date published: 2021-10-20

Is this compound molecular biology grade?

Asked by: SCchem16
Thank you for your question. This chemical, Tris base (CAS 77-86-1), Is not tested for Molecular biology grade. However, it is high quality and we suggest using Molecular biology grade solvents for filtering and testing a sample prior to use.
Answered by: Chemical Support 2
Date published: 2019-12-10
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Rated 5 out of 5 by from Solid and trustworthySolid and trustworthy. cant reccomend SCBT products enough
Date published: 2022-11-21
Rated 5 out of 5 by from Good productIt is cheaper than products from big companies such as Sigma. And it works for making buffers for biochemistry experiments we have
Date published: 2022-09-29
Rated 5 out of 5 by from Good reagentVery good reagent at a competitive price! We use it to prepare all the buffers we need!
Date published: 2022-07-15
Rated 5 out of 5 by from Easy order processReally easy order process. Very quick delivery also. Many thanks.
Date published: 2022-05-16
Rated 5 out of 5 by from Great price, reliable productVery good value, always reliable product from santa cruz
Date published: 2020-12-08
Rated 5 out of 5 by from 很好已经购买多次了，强烈推荐啊，感觉比国产的质量要好，但价格并不高啊！买了之后给个好评，还能得个优惠券！
Date published: 2020-10-01
Rated 5 out of 5 by from Excellent Customer CareAlways a pleasure to do business with SCTB. Great customer & technical service, timely shipment and quality chemicals.
Date published: 2019-06-28
Rated 5 out of 5 by from Tris-baseI bought this product a month ago and it is Excellent product!
Date published: 2019-05-15
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Tris base is rated 4.9 out of 5 by 16.
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