Trimethoprim-d9 (Major) (CAS 1189460-62-5)

Trimethoprim-d9, an isotopically labeled derivative of trimethoprim, is instrumental in scientific research, particularly in elucidating the pharmacokinetics, metabolism, and mechanism of action of trimethoprim, a broad-spectrum antibiotic. By substituting nine hydrogen atoms with deuterium, a stable isotope of hydrogen, trimethoprim-d9 enables precise tracking and quantification of the compound′s distribution and transformation in biological systems. Researchers utilize this labeled compound to investigate the interaction of trimethoprim with bacterial dihydrofolate reductase (DHFR), a key enzyme involved in folate metabolism. Trimethoprim-d9 serves as a valuable tool in structural biology studies, facilitating the determination of the binding mode and kinetics of trimethoprim to DHFR at the atomic level through techniques such as X-ray crystallography and nuclear magnetic resonance spectroscopy. Moreover, trimethoprim-d9 finds applications in microbial ecology research, where it aids in tracing the fate and transport of trimethoprim residues in environmental matrices, contributing to the understanding of antibiotic resistance dissemination and environmental contamination. Its use as a stable isotope tracer enhances the accuracy and reliability of pharmacokinetic studies and environmental monitoring efforts, ultimately advancing our knowledge of antibiotic action and resistance mechanisms in both clinical and environmental settings.