Trichosanthin Antibody (TCST-2): sc-69672

Trichosanthin Antibody (TCST-2) is a mouse monoclonal IgG1 antibody that detects Trichosanthin protein of non-species reactive by enzyme-linked immunosorbent assay (ELISA). Anti-Trichosanthin antibody (TCST-2) is available as a non-conjugated format. Trichosanthin, also referred to as TCS, is a type I ribosome-inactivating protein (RIP) predominantly found in the root tubers of the Chinese medicinal herb Trichosanthes kirilowii, commonly known as Tian Hua Fen. Trichosanthin plays a crucial role in cellular processes due to its ability to cleave supercoiled double-stranded DNA, resulting in the production of linear and nicked circular DNA. The structural integrity of Trichosanthin contains a single calcium metal ion per molecule, which is essential for endonucleolytic activity. Trichosanthin not only inhibits HIV-1 replication but also engages with endoplasmic reticulum-induced stress signaling pathways, leading to apoptosis in various tumor cell lines. Such interactions highlight Trichosanthin′s potential as a therapeutic agent in cancer treatment, emphasizing Trichosanthin′s importance in both antiviral and antitumor strategies.

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Trichosanthin Antibody (TCST-2) References:

1. Trichosanthin inhibits antigen-specific T cell expansion through nitric oxide-mediated apoptosis pathway.  |  Li, F., et al. 2005. Cell Immunol. 234: 23-30. PMID: 15967422
2. Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells.  |  Huang, H., et al. 2006. Life Sci. 79: 1287-92. PMID: 16725160
3. Trichosanthin induced Th2 polarization status.  |  Zhao, S., et al. 2006. Cell Mol Immunol. 3: 297-301. PMID: 16978539
4. Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses.  |  Chan, DS., et al. 2007. Nucleic Acids Res. 35: 1660-72. PMID: 17308345
5. Trichosanthin induced apoptosis in HL-60 cells via mitochondrial and endoplasmic reticulum stress signaling pathways.  |  Li, J., et al. 2007. Biochim Biophys Acta. 1770: 1169-80. PMID: 17570595
6. Trichosanthin-induced specific changes of cytoskeleton configuration were associated with the decreased expression level of actin and tubulin genes in apoptotic Hela cells.  |  Wang, P. and Li, JC. 2007. Life Sci. 81: 1130-40. PMID: 17881009
7. Trypanosoma cruzi: high ribosomal resistance to trichosanthin inactivation.  |  Juri Ayub, M., et al. 2008. Exp Parasitol. 118: 442-7. PMID: 17949717
8. Effect of site-directed PEGylation of trichosanthin on its biological activity, immunogenicity, and pharmacokinetics.  |  An, Q., et al. 2007. Biomol Eng. 24: 643-9. PMID: 18023612
9. The C-terminal end of P proteins mediates ribosome inactivation by trichosanthin but does not affect the pokeweed antiviral protein activity.  |  Ayub, MJ., et al. 2008. Biochem Biophys Res Commun. 369: 314-9. PMID: 18282466