TREX-2 Antibody (E-4): sc-390890

TREX-2 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TREX-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TREX-2 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TREX-2, also known as three prime repair exonuclease 2, is a crucial 279 amino acid enzyme that plays a significant role in maintaining genomic stability by participating in DNA repair processes. Located primarily in the nucleus, TREX-2 functions as an exonuclease, utilizing magnesium ions to catalyze the 3′ to 5′ exonucleolytic cleavage of mismatched double-stranded DNA, which is essential for correcting errors that may arise during DNA replication. This activity not only helps to eliminate mutant DNA but also generates nucleoside 5′ phosphates, thereby facilitating subsequent repair mechanisms. TREX-2 is expressed in various tissues, including the stomach, heart, breast, testis, prostate, colon, and skeletal muscle, and exists as a homodimer, which enhances enzymatic efficiency. Multiple isoforms of TREX-2 arise from alternative splicing, reflecting the complexity of regulation and function. The gene encoding human TREX-2 is located at Xq28 and is closely associated with the UCHL5IP gene, indicating potential co-regulation and functional interplay between these proteins.

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TREX-2 Antibody (E-4) References:

1. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3'-->5' exonucleases.  |  Mazur, DJ. and Perrino, FW. 1999. J Biol Chem. 274: 19655-60. PMID: 10391904
2. Structure and expression of the TREX1 and TREX2 3' --> 5' exonuclease genes.  |  Mazur, DJ. and Perrino, FW. 2001. J Biol Chem. 276: 14718-27. PMID: 11278605
3. The 3' 5' exonucleases.  |  Shevelev, IV. and Hübscher, U. 2002. Nat Rev Mol Cell Biol. 3: 364-76. PMID: 11988770
4. The TREX2 3'-->5' exonuclease physically interacts with DNA polymerase delta and increases its accuracy.  |  Shevelev, IV., et al. 2002. ScientificWorldJournal. 2: 275-81. PMID: 12806015
5. Sequence variants in the 3'-->5' deoxyribonuclease TREX2: identification in a genetic screen and effects on catalysis by the recombinant proteins.  |  Perrino, FW., et al. 2004. Adv Enzyme Regul. 44: 37-49. PMID: 15581481
6. The human TREX2 3' -> 5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis.  |  Perrino, FW., et al. 2005. J Biol Chem. 280: 15212-8. PMID: 15661738
7. Biochemical and cellular characteristics of the 3' -> 5' exonuclease TREX2.  |  Chen, MJ., et al. 2007. Nucleic Acids Res. 35: 2682-94. PMID: 17426129
8. Cooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonuclease.  |  Perrino, FW., et al. 2008. J Biol Chem. 283: 21441-52. PMID: 18534978

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TREX-2 Antibody (E-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TREX-2 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TREX-2 Antibody (E-4) Specifications

Alternate Product Name: TREX-2

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 248-268

Epitope Target: TREX-2

Epitope Species: human

Product Reactivity/Detection: TREX-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TREX-2 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin