TPPII Double Nickase Plasmid (m): sc-423485-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TPPII Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TPPII Double Nickase Plasmid (m) and TPPII Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tpp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPPII Double Nickase Plasmid (m) | sc-423485-NIC | 20 µg | $410.00 |
Tpp2 encodes tripeptidyl peptidase II (TPPII), a large cytosolic exopeptidase that trims proteasome-generated oligopeptides and contributes to intracellular protein turnover and amino acid homeostasis. TPPII activity interfaces with proteostasis networks, including ubiquitin–proteasome processing, cellular stress responses, and peptide handling relevant to antigen processing and presentation pathways. By shaping peptide pools and supporting protein quality control, TPPII can influence cell growth, survival signaling, and responses to proteotoxic stress. Dysregulated protease and proteostasis pathways that involve TPPII are frequently examined in models of inflammation, immune function, and disorders characterized by altered protein degradation.
TPPII Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tpp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tpp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tpp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tpp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.