TP53INP2 Double Nickase Plasmid (h): sc-405261-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TP53INP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TP53INP2 Double Nickase Plasmid (h) and TP53INP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TP53INP2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TP53INP2 Double Nickase Plasmid (h) | sc-405261-NIC | 20 µg | $410.00 | |||
TP53INP2 Double Nickase Plasmid (h2) | sc-405261-NIC-2 | 20 µg | $410.00 |
TP53INP2 (tumor protein p53 inducible nuclear protein 2) encodes a stress-responsive adaptor protein that coordinates autophagy and vesicular trafficking, with prominent roles in autophagosome formation and selective cargo handling. TP53INP2 interacts with autophagy machinery such as LC3/GABARAP family proteins and contributes to regulation of cellular homeostasis under nutrient deprivation and other stress conditions. Through these functions, TP53INP2 is connected to pathways controlling metabolism, protein turnover, and organelle quality control, processes frequently altered in cancer biology and inflammatory or neurodegenerative contexts. Altered TP53INP2 expression or function has been associated with dysregulated autophagy and cellular stress responses, supporting its study in mechanisms of survival, adaptation, and cell fate decisions.
TP53INP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TP53INP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TP53INP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TP53INP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TP53INP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.