Tom34 Antibody (S-05): sc-101284

Tom34 Antibody (S-05) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tom34 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Tom34 antibody (S-05) is available as a non-conjugated monoclonal isotype antibody. Tom34 protein, also known as TOMM34, URCC3, or HTOM34P, is a crucial component of the mitochondrial preprotein translocase complex, which is essential for importing nuclear-encoded precursor proteins into mitochondria. This 309 amino acid protein is characterized by six tetratricopeptide repeats (TPR), which play a significant role in protein-protein interactions. Located on the cytoplasmic side of the mitochondrial outer membrane, Tom34 (S-05) antibody recognizes a chaperone-like protein that binds to mature portions of unfolded proteins, facilitating proper import into mitochondria. This process is vital for maintaining mitochondrial function and cellular energy production, as mitochondria generate ATP through oxidative phosphorylation. Tom34 expression is upregulated in colon cancer cells, suggesting a potential role in tumor progression and making Tom34 a promising target for therapeutic anti-cancer strategies.

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Tom34 Antibody (S-05) References:

1. Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells.  |  Chewawiwat, N., et al. 1999. J Biochem. 125: 721-7. PMID: 10101285
2. Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins.  |  Mukhopadhyay, A., et al. 2002. Arch Biochem Biophys. 400: 97-104. PMID: 11913975
3. Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol.  |  Yang, CS. and Weiner, H. 2002. Arch Biochem Biophys. 400: 105-10. PMID: 11913976
4. Functional consequences of thyroid hormone-induced changes in the mitochondrial protein import pathway.  |  Colavecchia, M., et al. 2003. Am J Physiol Endocrinol Metab. 284: E29-35. PMID: 12388124
5. Compensatory responses of protein import and transcription factor expression in mitochondrial DNA defects.  |  Joseph, AM., et al. 2004. Am J Physiol Cell Physiol. 286: C867-75. PMID: 14656719
6. Conformation-sensitive gel electrophoresis as an ideal high-throughput strategy for accurate detection of sequence variations in DNA: screening hTomm and hTimm genes.  |  Blesa, JR., et al. 2004. J Biomol Screen. 9: 621-4. PMID: 15475482
7. Identification of TOMM34, which shows elevated expression in the majority of human colon cancers, as a novel drug target.  |  Shimokawa, T., et al. 2006. Int J Oncol. 29: 381-6. PMID: 16820880
8. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
9. NRF-1 is the major transcription factor regulating the expression of the human TOMM34 gene.  |  Blesa, JR., et al. 2008. Biochem Cell Biol. 86: 46-56. PMID: 18364745
10. Tom34: a cytosolic cochaperone of the Hsp90/Hsp70 protein complex involved in mitochondrial protein import.  |  Faou, P. and Hoogenraad, NJ. 2012. Biochim Biophys Acta. 1823: 348-57. PMID: 22178133
11. Overexpression of heat shock protein HSP90AA1 and translocase of the outer mitochondrial membrane TOM34 in HCV-induced hepatocellular carcinoma: A pilot study.  |  Toraih, EA., et al. 2019. Clin Biochem. 63: 10-17. PMID: 30521791
12. hTom34: a novel translocase for the import of proteins into human mitochondria.  |  Nuttall, SD., et al. 1997. DNA Cell Biol. 16: 1067-74. PMID: 9324309

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom34 Antibody (S-05) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom34 Antibody (S-05) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom34 Antibody (S-05) Specifications

Alternate Product Name: Tom34

Product Clone Name: S-05

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-310

Epitope Target: Tom34

Epitope Species: human

Product Reactivity/Detection: Tom34

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Tom34 Antibody (S-05): N/A