Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tom1 Double Nickase Plasmid (m): sc-423467-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tom1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tom1 Double Nickase Plasmid (m) and Tom1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tom1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tom1 Antibody (H-5): sc-514430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tom1 Double Nickase Plasmid (m) | sc-423467-NIC | 20 µg | $410.00 | |||
Tom1 Double Nickase Plasmid (m2) | sc-423467-NIC-2 | 20 µg | $410.00 |
Mouse Tom1 (target of Myb1) encodes an endosomal adaptor protein that binds ubiquitin and engages ESCRT-associated machinery to regulate trafficking and sorting of ubiquitinated membrane proteins. Through interactions with clathrin and endosomal components, TOM1 contributes to endosome maturation, cargo degradation, and control of receptor turnover, thereby influencing signaling amplitude and duration. TOM1 has also been linked to modulation of inflammatory signaling pathways, including regulation of IL-1/TLR axis via trafficking of signaling complexes. Dysregulated endolysosomal transport and innate immune signaling are relevant to neurodegenerative and immune-mediated disease mechanisms, making Tom1 a useful locus for pathway dissection in cellular models.
Tom1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tom1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tom1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tom1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tom1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.