TOCA-1 Double Nickase Plasmid (h): sc-408893-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TOCA-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TOCA-1 Double Nickase Plasmid (h) and TOCA-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FNBP1L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TOCA-1 Double Nickase Plasmid (h) | sc-408893-NIC | 20 µg | $410.00 | |||
TOCA-1 Double Nickase Plasmid (h2) | sc-408893-NIC-2 | 20 µg | $410.00 |
FNBP1L encodes TOCA-1, an F-BAR and SH3 domain–containing adaptor that couples membrane curvature sensing to actin cytoskeleton remodeling. TOCA-1 coordinates Cdc42-dependent recruitment and activation of N-WASP and the Arp2/3 complex, supporting clathrin-mediated endocytosis, vesicle trafficking, and formation of dynamic actin structures such as filopodia and invadopodia. Through these processes, TOCA-1 contributes to cell polarity, migration, and adhesion-dependent signaling in pathways linked to Rho GTPases and cortical actin regulation. Dysregulated TOCA-1–associated actin dynamics has been studied in contexts including altered cell motility and invasive behavior, making FNBP1L a useful target for mechanistic studies of cytoskeletal control.
TOCA-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FNBP1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FNBP1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FNBP1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FNBP1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.