TNF alpha Antibody (TN3-19.12): sc-12744

TNFα Antibody (TN3-19.12) is a hamster monoclonal IgG antibody that detects TNFα of mouse, rat, and rabbit origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TNFα Antibody (TN3-19.12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TNFα, also known as cachectin, is a critical cytokine involved in systemic inflammation and is produced by a variety of immune cells, including lymphocytes, neutrophils, and macrophages. TNFα can undergo post-translational modifications, such as glycosylation, which can influence stability, activity, and interactions with receptors. These modifications are essential for proper functioning of TNFα, as modifications can affect binding affinity to TNF receptors and modulate downstream signaling pathways that regulate immune responses and inflammation. Understanding these modifications is crucial for developing therapeutic strategies targeting TNFα in various diseases, including autoimmune disorders and cancer, where dysregulation can lead to pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TNF alpha Antibody (TN3-19.12) References:

1. Tumor necrosis factor alpha (TNF-α), anti-TNF-α and demyelination revisited: an ongoing story.  |  Caminero, A., et al. 2011. J Neuroimmunol. 234: 1-6. PMID: 21474190
2. The dual role of tumor necrosis factor-alpha (TNF-α) in breast cancer: molecular insights and therapeutic approaches.  |  Cruceriu, D., et al. 2020. Cell Oncol (Dordr). 43: 1-18. PMID: 31900901
3. -308G/A polymorphism of tumor necrosis factor alpha (TNF-α) gene and metabolic syndrome susceptibility: a meta-analysis.  |  Wang, D., et al. 2021. Sci Rep. 11: 3840. PMID: 33589701
4. The Role of Tumor Necrosis Factor Alpha (TNF-α) in Autoimmune Disease and Current TNF-α Inhibitors in Therapeutics.  |  Jang, DI., et al. 2021. Int J Mol Sci. 22: PMID: 33800290
5. Association between miR-146a and Tumor Necrosis Factor Alpha (TNF-α) in Stable Coronary Artery Disease.  |  Pereira-da-Silva, T., et al. 2021. Medicina (Kaunas). 57: PMID: 34199767
6. The Role of Tumor Necrosis Factor-α (TNF-α) Polymorphisms in Gastric Cancer: a Meta-Analysis.  |  Gholamalizadeh, M., et al. 2022. J Gastrointest Cancer. 53: 756-769. PMID: 34478034
7. Tumor necrosis factor alpha (TNF-α) blockage reduces acute inflammation and delayed wound healing in oral ulcer of rats.  |  Freitas, MO., et al. 2022. Inflammopharmacology. 30: 1781-1798. PMID: 35948810
8. Prospective Roles of Tumor Necrosis Factor-Alpha (TNF-α) in COVID-19: Prognosis, Therapeutic and Management.  |  Mohd Zawawi, Z., et al. 2023. Int J Mol Sci. 24: PMID: 37047115
9. Paradoxical Tumor Necrosis Factor-Alpha (TNF-α) Inhibitor-Induced Psoriasis: A Systematic Review of Pathogenesis, Clinical Presentation, and Treatment.  |  Chokshi, A., et al. 2023. Cureus. 15: e42791. PMID: 37664349
10. Cerebrospinal fluid tumor necrosis factor-alpha (TNF-α) levels in children with cerebral malaria.  |  Prasad, R., et al. 2023. J Trop Pediatr. 69: PMID: 37805828

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TNF alpha Antibody (TN3-19.12) has 45 citations in a variety of scientific publications.

Primary antibodies like Anti-TNF alpha Antibody (TN3-19.12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TNF alpha Antibody (TN3-19.12) Specifications

Alternate Product Name: TNF alpha

Product Clone Name: TN3-19.12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: TNFα

Epitope Species: mouse

Product Reactivity/Detection: TNFα

Species Reactivity/Detection: mouse, rat, rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 45 citations in various scientific publications

Available Conjugates/Forms for TNF alpha Antibody (TN3-19.12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred TNF alpha antibody is TNF alpha Antibody (52B83): sc-52746.