TNFα-IP 8L3 CRISPR Activation Plasmid (h): sc-410450-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNFα-IP 8L3 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- TNFα-IP 8L3 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by TNFα-IP 8L3 CRISPR Activation Plasmid (h) and TNFα-IP 8L3 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TNFAIP8L3 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNFα-IP 8L3 CRISPR Activation Plasmid (h) | sc-410450-ACT | 20 µg | $397.00 |
TNFAIP8L3 (TNFα-IP 8L3) is a member of the TNFAIP8/TIPE family implicated in regulating immune-related signaling, cell survival, and inflammatory responses downstream of cytokine stimulation. In human cells, TNFAIP8L3 has been linked to modulation of apoptotic thresholds and stress-adaptive pathways, with functional connections to NF-κB-associated transcriptional programs and broader TNF-driven network activity. Altered expression of TIPE family proteins has been reported across contexts involving dysregulated inflammation and oncogenic phenotypes, supporting investigation of TNFAIP8L3 in tumor microenvironment biology and immune cell signaling. Studying TNFAIP8L3 can help clarify how cytokine-responsive regulators shape proliferation, death signaling, and cellular homeostasis.
TNFα-IP 8L3 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TNFAIP8L3 expression without altering the underlying DNA sequence.
TNFα-IP 8L3 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TNFAIP8L3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TNFAIP8L3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous TNFα-IP 8L3 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TNFAIP8L3 locus and enabling the study of TNFα-IP 8L3-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of TNFα-IP 8L3 pathway restoration in tumor cells with silenced or reduced TNFAIP8L3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.