Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TMEM70 CRISPR/Cas9 KO Plasmid (h): sc-412399
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM70 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM70 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TMEM70 Antibody (B-6): sc-393619
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM70 CRISPR/Cas9 KO Plasmid (h) | sc-412399 | 20 µg | $397.00 |
Overview
TMEM70 encodes a mitochondrial inner membrane protein required for proper assembly and stabilization of oxidative phosphorylation complex V (ATP synthase), supporting efficient ATP production and mitochondrial energy homeostasis. Loss or dysfunction of TMEM70 perturbs proton-coupled ATP synthesis, often leading to altered mitochondrial membrane potential, disrupted respiratory chain function, and compensatory metabolic remodeling. TMEM70 is therefore a useful node for studying mitochondrial biogenesis, bioenergetics, and mitonuclear coordination in human cells. Pathogenic TMEM70 variants have been linked to autosomal recessive mitochondrial disease, including complex V deficiency with encephalocardiomyopathy and metabolic decompensation phenotypes, motivating mechanistic studies in relevant cell models.
TMEM70 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM70 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM70 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM70 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM70 protein expression.
This CRISPR knockout system enables efficient generation of TMEM70-deficient cell models for investigation of TMEM70 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM70 exon(s) critical for TMEM70 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM70 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM70 CRISPR/Cas9 KO Plasmid (h) and TMEM70 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM70 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM70 HDR Plasmid (h) and TMEM70 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM70 homology arms to support homology-directed repair at defined TMEM70 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.