TMEM171 Double Nickase Plasmid (h): sc-408549-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM171 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TMEM171 Double Nickase Plasmid (h) and TMEM171 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TMEM171. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM171 Double Nickase Plasmid (h) | sc-408549-NIC | 20 µg | $410.00 | |||
TMEM171 Double Nickase Plasmid (h2) | sc-408549-NIC-2 | 20 µg | $410.00 |
TMEM171 encodes a predicted multi-pass transmembrane protein thought to localize to intracellular membrane compartments and participate in membrane organization and trafficking-related processes. Although its molecular partners and signaling context remain incompletely defined, TMEM171 is studied as part of broader transmembrane protein networks that influence compartmentalization, vesicular transport, and cell-state regulation. Variation in TMEM171 expression has been explored in genomic and transcriptomic datasets across multiple tissue contexts, supporting ongoing investigation into potential links with dysregulated cellular homeostasis. These features make TMEM171 a useful target for mechanistic studies that connect membrane protein biology with downstream phenotypes.
TMEM171 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TMEM171 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TMEM171. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TMEM171 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TMEM171-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.