Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TLR9 Double Nickase Plasmid (h): sc-400600-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLR9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TLR9 Double Nickase Plasmid (h) and TLR9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TLR9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLR9 Antibody (5G5): sc-47723
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLR9 Double Nickase Plasmid (h) | sc-400600-NIC | 20 µg | $410.00 | |||
TLR9 Double Nickase Plasmid (h2) | sc-400600-NIC-2 | 20 µg | $410.00 |
Toll-like receptor 9 (TLR9) is an endosomal pattern-recognition receptor that detects unmethylated CpG motifs in microbial and self-derived DNA, initiating innate immune sensing. Upon ligand engagement and endosomal proteolytic processing, TLR9 signals primarily through the MyD88 adaptor to activate NF-κB and IRF transcriptional programs, driving inflammatory cytokines and type I interferon responses. This pathway contributes to antiviral and antibacterial immunity and shapes antigen-presenting cell function, including B cell activation. Dysregulated TLR9 signaling has been linked to autoinflammatory and autoimmune phenotypes and has been investigated in contexts such as systemic lupus erythematosus, psoriasis, and tumor-associated inflammation.
TLR9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TLR9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TLR9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TLR9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TLR9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.