Anti-TLR3 Antibody (TLR3.7) recommended for detection of TLR3 of human origin by WB, IF, FCM and ELISA

Anti-TLR3 Antibody (TLR3.7): sc-32232

Anti-TLR3 Antibody (TLR3.7) is rated 3.8 out of 5 by 13.
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Datasheets

    • Anti-TLR3 Antibody (TLR3.7) is a mouse monoclonal IgG1 κ TLR3 antibody, cited in 41 publications, provided at 200 µg/ml
    • raised against full length TLR3 of human origin
    • TLR3 Antibody (TLR3.7) is recommended for detection of TLR3 of human origin by WB, IF, FCM and ELISA
    • Anti-TLR3 Antibody (TLR3.7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR3 (TLR3.7): sc-32232.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TLR3 Antibody (TLR3.7) for WB applications. These reagents are now offered in bundles with TLR3 Antibody (TLR3.7) (see ordering information below).
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Does ot cross react with mouse TLR2 ?

Asked by: Akriti
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-12-11

Can TLR3 (TLR3.7): sc-32232 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TLR3 (TLR3.7): sc-32232 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well This antibody can be used in ovine lymph node for related research.
Date published: 2021-04-16
Rated 1 out of 5 by from FACS on HFF This antibody does not work on HFF cells in flow cytometry
Date published: 2018-11-01
Rated 1 out of 5 by from Funktioniert leider nicht bei mir im WB oder IF AK wurde in einer 1:200-Verdünnung im WB an stabil mit TLR3 transduzierten Hek-Zellen getestet. Leider konnte ich keine Bande in der richtigen Größe detektieren.
Date published: 2018-05-30
Rated 5 out of 5 by from Specific band obtained Tested on Human liver tissue (30ug) .There was a strong single band at the expected MW, great quality .Recommend used it in our labmates.
Date published: 2018-05-26
Rated 2 out of 5 by from Didn't work for me Used at 1:250 dilution in PBST 5% milk to detect in unstimulated A549 and HaCaT cells - didn't show anything but didn't have a positive control so may just be no TLR3 expressed at basal level in these cells
Date published: 2017-10-30
Rated 3 out of 5 by from My review for TLR3 antibody I applied for the sample sack a mouth ago. I used it to detect the TLR3 of human origin by Western Blotting (dilution 1:300, 1:200, 1:100). But I didn't detect the TLR3.
Date published: 2017-08-28
Rated 4 out of 5 by from Great IHC staining with paraffin-embedded tissue Great signal staining in IHC with paraffin-embedded gastric tissue
Date published: 2017-08-11
Rated 5 out of 5 by from It is really great The results of Western Blot analysis were very good, the band was clear
Date published: 2017-05-10
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