TLR2 (h): 293T Lysate: sc-115116

TLR2 (h): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express human Toll-like receptor 2 (TLR2). TLR2 is a crucial component of the innate immune system, recognizing pathogen-associated molecular patterns (PAMPs) from bacteria, fungi, and viruses. The 293T cell line is chosen for its high transfection efficiency and robust protein expression, making it an ideal system for producing TLR2 lysates. In research, TLR2 (h): 293T Lysate is used to study the molecular mechanisms of innate immune recognition and signaling. Upon binding to its ligands, TLR2 forms heterodimers with other TLRs, such as TLR1 or TLR6, initiating downstream signaling cascades. This activation leads to the recruitment of adaptor proteins like MyD88 and TIRAP, which subsequently activate NF-κB and MAPK pathways, resulting in the production of pro-inflammatory cytokines. Researchers employ TLR2 (h): 293T Lysate in biochemical assays such as Western blotting, immunoprecipitation, and luciferase reporter assays to analyze TLR2 expression, ligand binding, and downstream signaling events. This lysate is also used in functional studies to investigate the role of TLR2 in recognizing specific PAMPs and its involvement in the immune response to various pathogens. Additionally, TLR2 (h): 293T Lysate is valuable in screening assays to identify novel TLR2 agonists and antagonists, enhancing our understanding of TLR2-mediated signaling pathways and their regulation.