Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Thy-1/CD90 Double Nickase Plasmid (m): sc-423390-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Thy-1/CD90 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Thy-1/CD90 Double Nickase Plasmid (m) and Thy-1/CD90 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Thy1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Thy-1/CD90 Antibody (OX7): sc-53116
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thy-1/CD90 Double Nickase Plasmid (m) | sc-423390-NIC | 20 µg | $410.00 | |||
Thy-1/CD90 Double Nickase Plasmid (m2) | sc-423390-NIC-2 | 20 µg | $410.00 |
Thy1 encodes the glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein Thy-1/CD90, a conserved marker of neurons, fibroblasts, mesenchymal stromal cells, and subsets of T cells in mouse. Thy-1 participates in cell–cell and cell–matrix interactions through binding partners such as integrins and syndecans, influencing focal adhesion dynamics, cytoskeletal remodeling, and mechanotransduction. Through these processes it modulates neurite outgrowth, axon guidance, and immune cell activation, linking Thy-1 signaling to tissue remodeling and inflammatory cues. Dysregulated Thy-1 expression has been associated with fibrosis-like stromal activation, neuroinflammation, and tumor microenvironment phenotypes, making it relevant for studies of adhesion-dependent signaling and lineage identity.
Thy-1/CD90 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Thy1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Thy1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Thy1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Thy1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.