Thy-1/CD90 Antibody (FF-10): sc-59399
- Thy-1/CD90 Antibody (FF-10) is a rat monoclonal IgG1 provided at 200 µg/ml
- raised against EL-4 cells of mouse origin
- recommended for detection of Thy-1 on T cell lines (EL 4, 3A9), T lymphocytes, the WEH1-3 cell line and 3T3 fibroblasts of mouse origin by WB, IP, IF and FCM
- See Thy-1/CD90 (aTHy-1A1): sc-53456 for Thy-1/CD90 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Thy-1/CD90 Antibody (FF-10). This work is in progress.
QUICK LINKS
SEE ALSO...
Thy-1 Antibody (FF-10) is a rat monoclonal IgG1 antibody that detects Thy-1 in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Thy-1, also known as CDw90, is a phosphatidyl-anchored cell surface glycoprotein that plays a crucial role in cell adhesion and signaling, particularly in the context of hematopoietic stem cells. Expression of Thy-1 is vital for identifying specific subpopulations of cells within mixed colonies, especially when coexpressed with CD34, as Thy-1 marks a subset of putative hematopoietic, pluripotent stem cells in normal human bone marrow. Thy-1+ cells have been implicated in syngeneic graft versus host disease, highlighting Thy-1′s importance in regulating autoreactivity following bone marrow transplants. Understanding Thy-1 function is essential for advancing therapeutic strategies in regenerative medicine and immunology, as Thy-1 may influence stem cell behavior and interactions within the hematopoietic niche. Anti-Thy-1 antibody (FF-10) serves as an invaluable tool for researchers investigating these complex biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thy-1/CD90 Antibody (FF-10) References:
- Developmentally regulated expression of Thy-1 in structures of the mouse sensory-motor system. | Barlow, JZ. and Huntley, GW. 2000. J Comp Neurol. 421: 215-33. PMID: 10813783
- Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function. | Avalos, AM., et al. 2002. Biol Res. 35: 231-8. PMID: 12415741
- Thy-1 in hippocampus: normal anatomy and neuritic growth in Alzheimer's disease. | Leifer, D. and Kowall, NW. 1992. J Neuropathol Exp Neurol. 51: 133-41. PMID: 1347079
- Thy-1 regulates fibroblast focal adhesions, cytoskeletal organization and migration through modulation of p190 RhoGAP and Rho GTPase activity. | Barker, TH., et al. 2004. Exp Cell Res. 295: 488-96. PMID: 15093746
- Evolutionary analysis and expression of teleost Thy-1. | Reuter, A., et al. 2004. Zebrafish. 1: 191-201. PMID: 18248231
- Isolation and characterizaiton of murine cell surface components. I. Purification of milligram quantities of Thy-1.1. | Zwerner, RK., et al. 1977. J Exp Med. 146: 986-1000. PMID: 302318
- Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat. | Goldschneider, I., et al. 1978. J Exp Med. 148: 1351-66. PMID: 309914
- Expression of the Thy-1 glycoprotein gene by DNA-mediated gene transfer. | Evans, GA., et al. 1984. Proc Natl Acad Sci U S A. 81: 5532-6. PMID: 6147849
- Characterization of Thy-1 (CDw90) expression in CD34+ acute leukemia. | Holden, JT., et al. 1995. Blood. 86: 60-5. PMID: 7540889
- Retinal Thy-1 expression during development. | Liu, CJ., et al. 1996. Invest Ophthalmol Vis Sci. 37: 1469-73. PMID: 8641850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thy-1/CD90 Antibody (FF-10) | sc-59399 | 200 µg/ml | $322.00 |