Thrombin Antibody (F-1): sc-271449

Thrombin Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thrombin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Thrombin antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thrombin, also known as Coagulation Factor 2, plays a critical role in the hemostatic process following tissue injury by converting prothrombin into thrombin, which is essential for the formation of fibrin clots and platelet aggregation. Thrombin′s structure consists of a heavy chain and a light chain linked by a disulfide bond, which is crucial for enzymatic activity. This structural configuration allows Thrombin to effectively cleave fibrinogen into fibrin, thereby facilitating the formation of a stable clot. Thrombin′s ability to interact with various receptors on platelets and endothelial cells underscores its significance in regulating not only coagulation but also cellular responses during wound healing and tissue repair. Thrombin monoclonal antibody (F-1) enables researchers to gain valuable insights into the mechanisms of coagulation and the broader implications of Thrombin in pathological conditions such as cancer and cardiovascular diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Thrombin Antibody (F-1) References:

1. Thrombin inhibits tumor cell growth in association with up-regulation of p21(waf/cip1) and caspases via a p53-independent, STAT-1-dependent pathway.  |  Huang, YQ., et al. 2000. J Biol Chem. 275: 6462-8. PMID: 10692450
2. A phase 3, randomized, double-blind comparative study of the efficacy and safety of topical recombinant human thrombin and bovine thrombin in surgical hemostasis.  |  Chapman, WC., et al. 2007. J Am Coll Surg. 205: 256-65. PMID: 17660072
3. Detection of thrombin using electrogenerated chemiluminescence based on Ru(bpy)3(2+)-doped silica nanoparticle aptasensor via target protein-induced strand displacement.  |  Wang, X., et al. 2007. Anal Chim Acta. 598: 242-8. PMID: 17719898
4. Tyrosine phosphorylation / dephosphorylation balance is involved in thrombin-evoked microtubular reorganisation in human platelets.  |  Bouaziz, A., et al. 2007. Thromb Haemost. 98: 375-84. PMID: 17721620
5. Factor Xa-driven thrombin generation in plasma: dependency on the aminophospholipid density of membranes and inhibition by phospholipid-binding proteins.  |  Wielders, SJ., et al. 2007. Thromb Haemost. 98: 1056-62. PMID: 18000611
6. The coagulation cascade: initiation, maintenance, and regulation.  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
7. Basic mechanisms in blood coagulation.  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
8. Effects of thrombin and thrombin receptor activation on cardiac function after acute myocardial infarction.  |  Gu, X., et al. 2015. Am J Transl Res. 7: 654-69. PMID: 26064435
9. A method to measure thrombin activity in a mixture of fibrinogen and thrombin powders.  |  DeAnglis, AP., et al. 2017. Blood Coagul Fibrinolysis. 28: 134-138. PMID: 26991860
10. Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively.  |  Royle, NJ., et al. 1987. Somat Cell Mol Genet. 13: 285-92. PMID: 3474786
11. Thrombin stimulation of matrix fibronectin.  |  Armstrong, MT., et al. 1996. J Cell Physiol. 166: 112-20. PMID: 8557759
12. Thrombin stimulates fibroblast procollagen production via proteolytic activation of protease-activated receptor 1.  |  Chambers, RC., et al. 1998. Biochem J. 333 (Pt 1): 121-7. PMID: 9639571

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thrombin Antibody (F-1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Thrombin Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thrombin Antibody (F-1) Specifications

Alternate Product Name: Thrombin

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 331-376

Epitope Target: Thrombin

Epitope Species: human

Product Reactivity/Detection: Prothrombin precursor and mature chain, and Thrombin LC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Thrombin Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin