THP (m): 293T Lysate: sc-127653

THP (m): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express the mouse Tamm-Horsfall protein (THP), also known as uromodulin. THP is the most abundant protein in mammalian urine and is primarily produced by the epithelial cells of the thick ascending limb of the loop of Henle in the kidney. The 293T cell line, known for its high transfection efficiency and robust protein expression, is ideal for producing THP lysates. In research, THP (m): 293T Lysate is utilized to study the biochemical properties and physiological functions of THP. THP plays a crucial role in regulating water and salt balance, preventing urinary tract infections, and protecting against kidney stone formation. Mechanistically, THP is involved in modulating ion transport and inflammatory responses in the kidney. Researchers employ this lysate in various biochemical assays, including Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assays (ELISAs), to analyze THP expression levels, post-translational modifications, and interactions with other proteins. Additionally, THP (m): 293T Lysate is valuable in functional studies to investigate the effects of THP on renal cell function, ion transport, and immune responses. It is also used to explore the structural properties of THP, including its ability to form polymers and bind to various ligands. By utilizing THP (m): 293T Lysate, researchers gain insights into the regulatory mechanisms of THP and its role in maintaining kidney homeostasis and function.