THP-1 nuclear extract: sc-24963
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; monocytic leukemia cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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THP-1 nuclear extract is derived from the THP-1 cell line, a human monocytic cell line used extensively to study monocyte/macrophage functions, differentiation, and signaling pathways in a research context. This nuclear extract contains a variety of nuclear proteins, including transcription factors and other regulatory molecules critical for understanding the gene expression profiles and regulatory mechanisms in monocytes. THP-1 nuclear extract is particularly valuable for investigating the transcriptional responses to various stimuli that mimic infection or inflammation, providing insights into the cellular mechanisms governing immune responses. Researchers utilize this extract in applications such as chromatin immunoprecipitation (ChIP) to study the binding of transcription factors to DNA and assess how these interactions are altered under different treatment conditions. Additionally, electrophoretic mobility shift assays (EMSA) are performed to examine the DNA-binding activities of nuclear proteins, enhancing our understanding of the transcriptional regulation during monocyte to macrophage differentiation. The use of THP-1 nuclear extract allows for a detailed exploration of the intracellular signaling pathways and transcriptional networks involved in immune regulation. The origin and consistency of the THP-1 cell line are meticulously verified to ensure high fidelity in experimental outcomes, supporting its use in fundamental cellular and molecular biology research.
THP-1 nuclear extract References:
- Sp1 and Sp3 function as key regulators of leukotriene C(4) synthase gene expression in the monocyte-like cell line, THP-1. | Serio, KJ., et al. 2000. Am J Respir Cell Mol Biol. 23: 234-40. PMID: 10919991
- Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibit nuclear factor-kappa B-dependent gene activation at multiple levels in the human monocytic cell line THP-1. | Delgado, M. and Ganea, D. 2001. J Biol Chem. 276: 369-80. PMID: 11029467
- Transcriptional regulation of the human carboxyl ester lipase gene in THP-1 monocytes: an E-box required for activation binds upstream stimulatory factors 1 and 2. | Bengtsson, SH., et al. 2002. Biochem J. 365: 481-8. PMID: 11945176
- Opposing roles of activator protein-1 and CCAAT/enhancer binding protein beta in the regulation of inducible granulysin gene expression in a human monocytic cell line, THP-1. | Kida, Y., et al. 2002. Immunology. 107: 507-16. PMID: 12460196
- 5-lipoxygenase-activating protein gene expression. Key role of CCAAT/enhancer-binding proteins (C/EBP) in constitutive and tumor necrosis factor (TNF) alpha-induced expression in THP-1 cells. | Reddy, KV., et al. 2003. J Biol Chem. 278: 13810-8. PMID: 12571239
- A functional polymorphism in the Eta-1 promoter is associated with allele specific binding to the transcription factor Sp1 and elevated gene expression. | Hummelshoj, T., et al. 2006. Mol Immunol. 43: 980-6. PMID: 16009426
- A CRISPR/Cas9 approach reveals that the polymerase activity of DNA polymerase β is dispensable for HIV-1 infection in dividing and nondividing cells. | Goetze, RW., et al. 2017. J Biol Chem. 292: 14016-14025. PMID: 28684413
- Cyclic AMP signaling pathways are important in IL-1 beta transcriptional regulation. | Chandra, G., et al. 1995. J Immunol. 155: 4535-43. PMID: 7594450
- Lipopolysaccharide induction of tissue factor expression in THP-1 monocytic cells. Protein-DNA interactions with the promoter. | Donovan-Peluso, M., et al. 1994. J Biol Chem. 269: 1361-9. PMID: 8288602
- Repression by a differentiation-specific factor of the human cytomegalovirus enhancer. | Huang, TH., et al. 1996. Nucleic Acids Res. 24: 1695-701. PMID: 8649988
- Involvement of an SAF-like transcription factor in the activation of serum amyloid A gene in monocyte/macrophage cells by lipopolysaccharide. | Ray, BK. and Ray, A. 1997. Biochemistry. 36: 4662-8. PMID: 9109677
- Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with two transcription factors, nuclear factor-interleukin-6 and Spi-1. | Tsukada, J., et al. 1997. Blood. 90: 3142-53. PMID: 9376596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THP-1 nuclear extract | sc-24963 | 250 µg/0.05 ml | $163.00 |