TFIID/TATA Binding Protein/TBP Antibody (58C9): sc-421

TFIID [TBP] Antibody (58C9) is a mouse monoclonal IgG2b antibody that detects TFIID protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TFIID [TBP] (58C9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. TFIID protein plays a crucial role in the initiation of transcription in eukaryotic cells, serving as a key component of the transcription machinery that assembles at the core promoter region. TFIID recognizes and binds to the TATA box, a conserved DNA sequence located approximately 25-30 base pairs upstream of the transcription start site, which is essential for the recruitment of RNA polymerase II and other transcription factors. TATA-binding protein (TBP), a subunit of TFIID, contains a highly conserved C-terminal domain that is critical for function, as TBP facilitates the bending of DNA, allowing for proper assembly of the transcription pre-initiation complex. This process is vital for accurate expression of protein-coding genes, making TFIID an essential player in gene regulation and cellular function.

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TFIID/TATA Binding Protein/TBP Antibody (58C9) References:

1. TFIIA induces conformational changes in TFIID via interactions with the basic repeat.  |  Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
2. Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau).  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
3. Action of transcription factors in the control of transferrin receptor expression in human brain endothelium.  |  Holloway, K., et al. 2007. J Mol Biol. 365: 1271-84. PMID: 17125792
4. Structural analysis and dimerization potential of the human TAF5 subunit of TFIID.  |  Bhattacharya, S., et al. 2007. Proc Natl Acad Sci U S A. 104: 1189-94. PMID: 17227857
5. Identification of a small TAF complex and its role in the assembly of TAF-containing complexes.  |  Demény, MA., et al. 2007. PLoS One. 2: e316. PMID: 17375202
6. Crystal structure, biochemical and genetic characterization of yeast and E. cuniculi TAF(II)5 N-terminal domain: implications for TFIID assembly.  |  Romier, C., et al. 2007. J Mol Biol. 368: 1292-306. PMID: 17397863
7. A TATA binding protein regulatory network that governs transcription complex assembly.  |  Huisinga, KL. and Pugh, BF. 2007. Genome Biol. 8: R46. PMID: 17407552
8. Structure and functional properties of human general transcription factor IIE.  |  Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
9. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.  |  Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
10. Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3.  |  Farrelly, LA., et al. 2019. Nature. 567: 535-539. PMID: 30867594
11. RNA polymerase II transcription with partially assembled TFIID complexes.  |  Hisler, V., et al. 2023. bioRxiv.. PMID: 38076793
12. TAF2, within the TFIID complex, regulates the expression of a subset of protein-coding genes.  |  Cheng, IH., et al. 2024. Cell Death Discov. 10: 244. PMID: 38773077

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) has 82 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIID/TATA Binding Protein/TBP Antibody (58C9) Specifications

Alternate Product Name: TFIID [TBP]

Product Clone Name: 58C9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: TFIID

Epitope Species:

Product Reactivity/Detection: TATA binding protein (TBP)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Drosophila

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 82 citations in various scientific publications

Available Conjugates/Forms for TFIID/TATA Binding Protein/TBP Antibody (58C9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent