TFE3 Double Nickase Plasmid (h): sc-401179-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFE3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFE3 Double Nickase Plasmid (h) and TFE3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TFE3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFE3 Double Nickase Plasmid (h) | sc-401179-NIC | 20 µg | $410.00 | |||
TFE3 Double Nickase Plasmid (h2) | sc-401179-NIC-2 | 20 µg | $410.00 |
TFE3 (transcription factor binding to IGHM enhancer 3) is a MiT/TFE family bHLH-Zip transcription factor that regulates programs controlling lysosomal biogenesis, autophagy, and cellular metabolism. It integrates signals from nutrient-sensing pathways, including mTORC1-dependent regulation of subcellular localization, to coordinate transcriptional responses to stress and starvation. In the nucleus, TFE3 can induce genes involved in lysosomal function and catabolic flux, shaping processes such as endolysosomal trafficking and mitochondrial homeostasis. Dysregulated TFE3 activity is implicated in disease-relevant contexts including TFE3 gene fusions in renal cell carcinoma and altered transcriptional control of proteostasis and inflammatory signaling.
TFE3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TFE3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TFE3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TFE3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TFE3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.