TFE3 CRISPR Activation Plasmid (h): sc-401179-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFE3 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- TFE3 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by TFE3 CRISPR Activation Plasmid (h) and TFE3 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TFE3 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFE3 CRISPR Activation Plasmid (h) | sc-401179-ACT | 20 µg | $397.00 |
TFE3 (transcription factor E3) is a MiT/TFE family basic helix–loop–helix leucine zipper transcription factor that binds E-box motifs to coordinate programs controlling lysosomal biogenesis, autophagy, and cellular metabolism. It integrates nutrient and stress signaling through interactions with mTORC1-dependent regulation and cooperates with TFEB/TFE3-driven lysosomal gene networks to modulate vesicle trafficking and oxidative metabolism. In human disease biology, altered TFE3 activity is linked to dysregulated proteostasis and innate immune responses, and TFE3 gene fusions or overexpression are recurrent in subsets of renal cell carcinoma and other neoplasms. These properties make TFE3 a useful node for dissecting transcriptional control of catabolic pathways and organelle homeostasis.
TFE3 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TFE3 expression without altering the underlying DNA sequence.
TFE3 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TFE3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TFE3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous TFE3 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TFE3 locus and enabling the study of TFE3-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of TFE3 pathway restoration in tumor cells with silenced or reduced TFE3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.