TESK2 CRISPR/Cas9 KO Plasmid (m): sc-432964
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TESK2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TESK2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TESK2 CRISPR/Cas9 KO Plasmid (m) | sc-432964 | 20 µg | $397.00 |
Overview
Testis-associated kinase 2 (TESK2), encoded by the mouse Tesk2 gene, is a serine/threonine kinase that regulates actin cytoskeleton dynamics by phosphorylating and inhibiting cofilin, thereby influencing filament turnover, cell shape, and motility. TESK2 activity connects upstream signaling cues, including Rho-family GTPase pathways, to cytoskeletal remodeling processes that impact adhesion, migration, and vesicular trafficking. Expression is enriched in testicular tissues and germ cells, supporting roles in spermatogenesis and cytoskeletal reorganization during germ cell maturation. Dysregulated TESK2 signaling and cofilin-dependent actin remodeling are commonly examined in models of altered cell migration and tissue architecture, providing relevance to mechanistic studies of developmental and proliferative disorders.
TESK2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tesk2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tesk2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tesk2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TESK2 protein expression.
This CRISPR knockout system enables efficient generation of Tesk2-deficient cell models for investigation of TESK2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tesk2 exon(s) critical for TESK2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tesk2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TESK2 CRISPR/Cas9 KO Plasmid (m) and TESK2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tesk2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TESK2 HDR Plasmid (m) and TESK2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tesk2 homology arms to support homology-directed repair at defined Tesk2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.