TCR V β 8.5 Antibody (3H2990): sc-73131

TCR V β 8.5 Antibody (3H2990) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCR V beta 8.5 protein of mouse and rat origin by flow cytometry (FCM). Anti-TCR V β 8.5 antibody (3H2990) is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling pathways that activate T cells, transitioning them from a resting state to an active one. The TCR is a heterodimer composed of either alpha and beta or gamma and delta chains, with approximately 95% of circulating T cells expressing the alpha/beta heterodimer. This diversity in TCR specificity is essential for the immune system to effectively recognize a wide array of antigens, achieved through the somatic recombination of variable (V), diversity (D), and joining (J) gene segments during T cell development. The genes encoding the TCR beta and gamma chains are located in distinct loci, while the alpha and delta chain genes are found within a single locus. Notably, the synthesis of the TCR beta chain involves a complex process of joining D and J segments, followed by the addition of a V segment, culminating in the incorporation of a constant (C) segment. Understanding the structure and function of the TCR is vital, as genetic mutations in the TCR beta locus have been linked to the development of T cell lymphomas, highlighting TCR V β 8.5 (3H2990) antibody′s importance in both basic research and clinical applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TCR V β 8.5 Antibody (3H2990) References:

1. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.  |  Behlke, MA., et al. 1987. J Exp Med. 165: 257-62. PMID: 2432150
2. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.  |  Staerz, UD., et al. 1985. J Immunol. 134: 3994-4000. PMID: 2580901
3. Methods for the selection and growth of antigen-specific cytolytic T lines and clones bearing a defined T cell receptor beta chain marker.  |  Ito, M., et al. 1987. J Immunol Methods. 103: 229-37. PMID: 2959725
4. The inflammatory lesion of T cell line transferred experimental autoimmune encephalomyelitis of the Lewis rat: distinct nature of parenchymal and perivascular infiltrates.  |  Lannes-Vieira, J., et al. 1994. Acta Neuropathol. 87: 435-42. PMID: 7520206
5. T-cell receptor (TCR) usage in Lewis rat experimental autoimmune encephalomyelitis: TCR beta-chain-variable-region V beta 8.2-positive T cells are not essential for induction and course of disease.  |  Gold, R., et al. 1995. Proc Natl Acad Sci U S A. 92: 5850-4. PMID: 7597040
6. Preferential distribution of V beta 8.2-positive T cells in the central nervous system of rats with myelin basic protein-induced autoimmune encephalomyelitis.  |  Tsuchida, M., et al. 1993. Eur J Immunol. 23: 2399-406. PMID: 7691605
7. A mouse monoclonal antibody specific for the V beta 5.3 chain of the human TcR recognizes a subgroup of the mouse TcR V beta 8.2 chains.  |  Bleux, C., et al. 1995. J Leukoc Biol. 57: 491. PMID: 7884322
8. Dominant V beta 8 gene usage in response to TNP: failure to use other V beta chains following removal of V beta 8+ T cells by monoclonal antibody in vivo.  |  Dieli, F., et al. 1994. Immunology. 82: 99-105. PMID: 8045598
9. T cell vaccination against autoimmune diabetes in nonobese diabetic mice.  |  Formby, B. and Shao, T. 1993. Ann Clin Lab Sci. 23: 137-47. PMID: 8096128
10. Involvement of TCR-V beta 8.3+ cells in the cure of mice bearing a large MOPC-315 tumor by low dose melphalan.  |  Mokyr, MB., et al. 1993. J Immunol. 151: 4838-46. PMID: 8409442
11. Analysis of heart-infiltrating T-cell clonotypes in experimental autoimmune myocarditis in rats.  |  Hanawa, H., et al. 1996. Circ Res. 78: 118-25. PMID: 8603494
12. Microbial colonization influences composition and T-cell receptor V beta repertoire of intraepithelial lymphocytes in rat intestine.  |  Helgeland, L., et al. 1996. Immunology. 89: 494-501. PMID: 9014812

The preferred TCR β antibody is TCR β Antibody (G-11): sc-5277.